Heterogeneity of bromodeoxyuridine sensitivity of cultured cells from melanoma metastases.
Heterogeneity of bromodeoxyuridine sensitivity of cultured cells from melanoma metastases.
复制标题
黑色素瘤转移培养细胞对溴脱氧尿苷敏感性的异质性。
DOI：
10.1002/cyto.990210112
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
H. Hoehn
中科院分区：
文献类型：
--
作者：
M. Poot;K. Hiller;S. Heimpel;A. Schuster;J. Köhler;H. Hoehn
持续生长的细胞培养物，酪氨酸活性检测呈阳性，来自5名恶性黑色素瘤患者的2个脑和3个淋巴结转移灶。用连续溴脱氧尿苷（BrdUrd）标记，然后用双变量Hoechst 33258/溴化乙啶流式细胞术分析这些细胞培养物的增殖率。与人类二倍体成纤维细胞培养物相比，黑色素瘤细胞培养物对BrdUrd更敏感：成纤维细胞在360 +/- 130 microM BrdUrd（范围：130-520；n = 11）和650 +/- 50 microM BrdUrd （n = 3）下的生长抑制率为50%。此外，BrdUrd在黑色素瘤细胞中的敏感性是氧气依赖性的：20%氧气条件下，200 +/- 55微米（范围：65-400微米）的生长抑制率为50%，而5%氧气条件下，BrdUrd为360 +/- 130微米。brdurd诱导生长抑制的细胞周期动力学机制是细胞在G2期的积累。单个转移灶的培养显示BrdUrd敏感性的3倍变异。在一个脑转移中，两个不同倍性水平的群体（假三倍体和假四倍体）和BrdUrd敏感性可以解决。因此，连续BrdUrd标记和双变量Hoechst 33258/溴化乙醚流式细胞术是检测肿瘤活检中细胞群增殖能力和药物敏感性异质性的有力工具。
Continuously growing cell cultures, testing positive for tyrosine activity, were derived from two brain and three lymph-node metastases of five patients with malignant melanoma. These cell cultures were analyzed regarding their proliferation rate with continuous bromodeoxyuridine (BrdUrd) labeling followed by bivariate Hoechst 33258/ethidium bromide flow cytometry. Melanoma cell cultures are more sensitive toward BrdUrd in comparison to human diploid fibroblast cultures: 50% growth inhibition at 360 +/- 130 microM BrdUrd (range: 130-520; n = 11) vs. 650 +/- 50 microM BrdUrd (n = 3) for fibroblasts. Moreover, BrdUrd sensitivity in melanoma cells is oxygen dependent: 50% growth inhibition at 200 +/- 55 microM (range: 65-400 microM) for 20% oxygen vs. 360 +/- 130 microM BrdUrd for 5% oxygen. The cell cycle kinetic mechanisms of BrdUrd-induced growth inhibition is accumulation of cells in the G2 phase. Cultures from a single metastasis showed up to a 3-fold variation in BrdUrd sensitivity. In one of the brain metastases two populations of different ploidy level (pseudotriploid vs. pseudotetraploid) and BrdUrd sensitivity could be resolved. Thus, continuous BrdUrd labeling followed by bivariate Hoechst 33258/ethidium bromide flow cytometry is a powerful tool to detect heterogeneity in proliferative capacity and drug sensitivity of cell populations within one tumor biopsy.
登录
查看更多内容
溴脱氧尿苷会放大自由基介导的 DNA 损伤。
DOI：
10.1042/bj2610269
发表时间：
1989
期刊：
The Biochemical journal
影响因子：
--
作者：
Poot,M;Rabinovitch,PS;Hoehn,H
通讯作者：
Hoehn,H
影响因子：
11.2
作者：
I. Fidler
通讯作者：
I. Fidler
黑色素瘤中的染色体和生长因子异常。
影响因子：
--
作者：
Priest,JH;Phillips,CN;Wang,Y;Richmond,A
通讯作者：
Richmond,A
DOI：
10.1073/pnas.80.10.2951
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
RABINOVITCH, PS
通讯作者：
RABINOVITCH, PS
影响因子：
3.7
作者：
RABINOVITCH, PS;KUBBIES, M;HOEHN, H
通讯作者：
HOEHN, H