Evaluation of toxicity of the mycotoxin citrinin using yeast ORF DNA microarray and Oligo DNA microarray.
Evaluation of toxicity of the mycotoxin citrinin using yeast ORF DNA microarray and Oligo DNA microarray.
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DOI：
10.1186/1471-2164-8-95
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发表时间：
2007-04-05
期刊：
影响因子：
4.4
通讯作者：
Iwahashi Y
中科院分区：
文献类型：
--
作者：
Iwahashi H;Kitagawa E;Suzuki Y;Ueda Y;Ishizawa YH;Nobumasa H;Kuboki Y;Hosoda H;Iwahashi Y
真菌毒素是常见于饲料和食品中的真菌次级代谢产物，被广泛认为是有害污染物。桔霉素是最早从桔青霉中分离出来的一种真菌毒素，由10多种真菌产生，可能分布于世界各地。然而，关于该毒素的作用机制的信息是有限的。因此，我们研究了桔霉素诱导的基因组反应，以评估其毒性。桔霉素在高于100 ppm的浓度下抑制酵母细胞的生长。我们利用ORF基因芯片和Oligo基因芯片检测了桔霉素诱导的酵母mRNA表达谱，并与其他胁迫诱导剂的表达谱进行了比较。从两个微阵列实验中获得的结果聚集在一起，但与霉菌毒素展青霉素的结果不同。氧化应激反应基因AAD、FLR1、OYE3、GRE2和MET 17被显著诱导。在功能类别中，参与“代谢”、“细胞拯救、防御和毒力”以及“能量”的基因表达被显着激活。在“代谢”类别中，参与谷胱甘肽合成途径的基因被激活，在“细胞拯救、防御和毒力”类别中，ABC转运蛋白基因被诱导。为了减轻诱导的应激，这些细胞可能在用谷胱甘肽修饰后泵出桔霉素。而桔霉素处理没有诱导参与DNA修复的基因。两个微阵列研究的结果表明，桔霉素治疗诱导酵母细胞的氧化应激。遗传毒性比棒曲霉素严重，表明桔霉素的毒性比棒曲霉素低。用寡核苷酸基因芯片检测表达谱的重复性更好。然而，Oligo DNA微阵列并没有完全克服交叉杂交。
Mycotoxins are fungal secondary metabolites commonly present in feed and food, and are widely regarded as hazardous contaminants. Citrinin, one of the very well known mycotoxins that was first isolated from Penicillium citrinum, is produced by more than 10 kinds of fungi, and is possibly spread all over the world. However, the information on the action mechanism of the toxin is limited. Thus, we investigated the citrinin-induced genomic response for evaluating its toxicity. Citrinin inhibited growth of yeast cells at a concentration higher than 100 ppm. We monitored the citrinin-induced mRNA expression profiles in yeast using the ORF DNA microarray and Oligo DNA microarray, and the expression profiles were compared with those of the other stress-inducing agents. Results obtained from both microarray experiments clustered together, but were different from those of the mycotoxin patulin. The oxidative stress response genes – AADs, FLR1, OYE3, GRE2, and MET17 – were significantly induced. In the functional category, expression of genes involved in "metabolism", "cell rescue, defense and virulence", and "energy" were significantly activated. In the category of "metabolism", genes involved in the glutathione synthesis pathway were activated, and in the category of "cell rescue, defense and virulence", the ABC transporter genes were induced. To alleviate the induced stress, these cells might pump out the citrinin after modification with glutathione. While, the citrinin treatment did not induce the genes involved in the DNA repair. Results from both microarray studies suggest that citrinin treatment induced oxidative stress in yeast cells. The genotoxicity was less severe than the patulin, suggesting that citrinin is less toxic than patulin. The reproducibility of the expression profiles was much better with the Oligo DNA microarray. However, the Oligo DNA microarray did not completely overcome cross hybridization.
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影响因子：
3.5
作者：
Yu, FY;Liao, YC;Liu, BH
通讯作者：
Liu, BH
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Lettieri T
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