Recent applications of DNA microarray technology to toxicology and ecotoxicology.
Recent applications of DNA microarray technology to toxicology and ecotoxicology.
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DOI：
10.1289/ehp.8194
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发表时间：
2006-01
影响因子：
10.4
通讯作者：
Lettieri T
中科院分区：
文献类型：
--
作者：
Lettieri T
基因表达是表征细胞和生物体如何适应外部环境变化的一种独特方式。在接触某种化学物质时对基因表达水平的测量，既可以用来提供有关该有毒物质作用机制的信息，也可以用来形成一种“遗传特征”，以确定有毒产物。高质量、商业上可用的基因阵列的发展使得这项技术成为分子毒理学的标准工具。若干国家和国际倡议为基因表达应用于研究新化合物和现有化合物的毒性提供了原理证明试验。在过去的几年里，该领域已经从评估技术的潜力发展到说明基因表达谱在毒理学中的实际应用。基因表达谱在生态毒理学中的应用尚处于较早阶段，主要是因为在分析自然种群状态时涉及许多变量。然而，对模式生物和非模式生物对环境应激源的反应进行了大量的研究。可以预见，生态毒理学基因表达谱中应激源特异性特征的发展将在不久的将来对生态毒理学领域产生重大影响。国际合作可以在加速基因组学方法在生态毒理学中的应用方面发挥重要作用。
Gene expression is a unique way of characterizing how cells and organisms adapt to changes in the external environment. The measurements of gene expression levels upon exposure to a chemical can be used both to provide information about the mechanism of action of the toxicant and to form a sort of “genetic signature” for the identification of toxic products. The development of high-quality, commercially available gene arrays has allowed this technology to become a standard tool in molecular toxicology. Several national and international initiatives have provided the proof-of-principle tests for the application of gene expression for the study of the toxicity of new and existing chemical compounds. In the last few years the field has progressed from evaluating the potential of the technology to illustrating the practical use of gene expression profiling in toxicology. The application of gene expression profiling to ecotoxicology is at an earlier stage, mainly because of the the many variables involved in analyzing the status of natural populations. Nevertheless, significant studies have been carried out on the response to environmental stressors both in model and in nonmodel organisms. It can be easily predicted that the development of stressor-specific signatures in gene expression profiling in ecotoxicology will have a major impact on the ecotoxicology field in the near future. International collaborations could play an important role in accelerating the application of genomic approaches in ecotoxicology.
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影响因子：
12.3
作者：
Fay JC;McCullough HL;Sniegowski PD;Eisen MB
通讯作者：
Eisen MB
影响因子：
3.8
作者：
Hamadeh, HK;Bushel, PR;Afshari, CA
通讯作者：
Afshari, CA
影响因子：
56.9
作者：
Armbrust, EV;Berges, JA;Rokhsar, DS
通讯作者：
Rokhsar, DS
影响因子：
2
作者：
Ikeo, K;Ishi-i, J;Tateno, Y
通讯作者：
Tateno, Y
DOI：
10.1073/pnas.98.4.1993
发表时间：
2001-02-13
影响因子：
11.1
作者：
Gracey, AY;Troll, JV;Somero, GN
通讯作者：
Somero, GN