MoPex19, which is essential for maintenance of peroxisomal structure and woronin bodies, is required for metabolism and development in the rice blast fungus.
MoPex19, which is essential for maintenance of peroxisomal structure and woronin bodies, is required for metabolism and development in the rice blast fungus.
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DOI：
10.1371/journal.pone.0085252
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Sun G
中科院分区：
文献类型：
--
作者：
Li L;Wang J;Zhang Z;Wang Y;Liu M;Jiang H;Chai R;Mao X;Qiu H;Liu F;Sun G
过氧化物酶体在真核生物中广泛存在，在细胞代谢中起着重要作用。它们在植物真菌病原体的致病性中起着至关重要的作用。过氧化物酶体基质蛋白和过氧化物酶体膜蛋白（pmp）在细胞质中合成并在翻译后输入。虽然过氧化物酶体的输入机制通常是保守的，但在不同类型的生物中发现了一些物种特有的特征。在植物病原真菌中，基质蛋白的途径已经被阐明，而pmp的进口机制仍然不清楚。本研究报道，作为ScPEX19的同源基因，MoPEX19是稻瘟病菌pmp导入和过氧化物酶体维持所必需的，在稻瘟病菌的代谢和致病性中起着至关重要的作用。MoPEX19低表达，Mopex19p分布于细胞质和新形成的过氧化物酶体中。MoPEX19的缺失导致过氧化物酶体膜蛋白（pmp）以及过氧化物酶体基质蛋白的错误定位。过氧化物酶体结构在Δmopex19突变体中完全缺失，woronin体也消失了。Δmopex19表现出过氧化物酶体疾病中典型的代谢缺陷，以及在已知的mopex突变体中未检测到的乙醛酸循环异常。Δmopex19突变体在真菌发育和致病性相关的形态发生方面出现了多种紊乱，完全丧失了对宿主的致病性。这些数据表明，在稻瘟病菌中，MoPEX19在维持过氧化物酶体和过氧化物酶体衍生结构中起着至关重要的作用，并且比已知的MoPEX基因对真菌发育和致病性的贡献更大。
Peroxisomes are present ubiquitously and make important contributions to cellular metabolism in eukaryotes. They play crucial roles in pathogenicity of plant fungal pathogens. The peroxisomal matrix proteins and peroxisomal membrane proteins (PMPs) are synthesized in the cytosol and imported post-translationally. Although the peroxisomal import machineries are generally conserved, some species-specific features were found in different types of organisms. In phytopathogenic fungi, the pathways of the matrix proteins have been elucidated, while the import machinery of PMPs remains obscure. Here, we report that MoPEX19, an ortholog of ScPEX19, was required for PMPs import and peroxisomal maintenance, and played crucial roles in metabolism and pathogenicity of the rice blast fungus Magnaporthe oryzae. MoPEX19 was expressed in a low level and Mopex19p was distributed in the cytoplasm and newly formed peroxisomes. MoPEX19 deletion led to mislocalization of peroxisomal membrane proteins (PMPs), as well peroxisomal matrix proteins. Peroxisomal structures were totally absent in Δmopex19 mutants and woronin bodies also vanished. Δmopex19 exhibited metabolic deficiency typical in peroxisomal disorders and also abnormality in glyoxylate cycle which was undetected in the known mopex mutants. The Δmopex19 mutants performed multiple disorders in fungal development and pathogenicity-related morphogenesis, and lost completely the pathogenicity on its hosts. These data demonstrate that MoPEX19 plays crucial roles in maintenance of peroxisomal and peroxisome-derived structures and makes more contributions to fungal development and pathogenicity than the known MoPEX genes in the rice blast fungus.
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影响因子：
--
作者：
Hynes, Michael J.;Murray, Sandra L.;Davis, Meryl A.
通讯作者：
Davis, Meryl A.
影响因子：
6
作者：
Bonekamp, Nina A.;Voelkl, Alfred;Schrader, Michael
通讯作者：
Schrader, Michael
DOI：
10.1083/jcb.135.1.1
发表时间：
1996-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Distel B;Erdmann R;Gould SJ;Blobel G;Crane DI;Cregg JM;Dodt G;Fujiki Y;Goodman JM;Just WW;Kiel JA;Kunau WH;Lazarow PB;Mannaerts GP;Moser HW;Osumi T;Rachubinski RA;Roscher A;Subramani S;Tabak HF;Tsukamoto T;Valle D;van der Klei I;van Veldhoven PP;Veenhuis M
通讯作者：
Veenhuis M
影响因子：
56.9
作者：
HAMER, JE;HOWARD, RJ;VALENT, B
通讯作者：
VALENT, B
影响因子：
3.5
作者：
Jungwirth, Helmut;Ring, Julia;Madeo, Frank
通讯作者：
Madeo, Frank