Coupling Novel Probes with Molecular Localization Microscopy Reveals Cell Wall Homeostatic Mechanisms in Staphylococcus aureus.
Coupling Novel Probes with Molecular Localization Microscopy Reveals Cell Wall Homeostatic Mechanisms in Staphylococcus aureus.
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DOI：
10.1021/acschembio.2c00741
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发表时间：
2022-12-16
影响因子：
4
通讯作者：
Foster, Simon J.
中科院分区：
文献类型：
--
作者：
Lund, Victoria A.;Gangotra, Haneesh;Zhao, Zhen;Sutton, Joshua A. F.;Wacnik, Katarzyna;DeMeester, Kristen;Liang, Hai;Santiago, Cintia;Grimes, Catherine Leimkuhler;Jones, Simon;Foster, Simon J.
细菌细胞壁肽聚糖是生存所必需的，其合成是抗生素（包括青霉素）的靶向。为了确定肽聚糖稳态如何控制细胞的结构、生长和分裂，我们开发了新的标记方法。这些与超分辨率荧光显微镜兼容，以检查肽聚糖合成，水解及其生物合成所需的酶（青霉素结合蛋白（PBPs））的定位。基于头孢菌素的荧光探针的合成揭示了分裂期间隔膜处的PBP模式，支持分散的肽聚糖合成的模型。代谢和羟胺为基础的探针分别使合成的聚糖链和相关的肽聚糖的还原末端被映射。还原末端的焦点和弧出现作为聚糖链的合成和主要肽聚糖水解酶SagB的氨基葡糖苷酶活性的结果。我们的研究提供了在生长和分裂过程中如何控制必需肽聚糖动力学的分子水平细节。
Bacterial cell wall peptidoglycan is essential for viability, and its synthesis is targeted by antibiotics, including penicillin. To determine how peptidoglycan homeostasis controls cell architecture, growth, and division, we have developed novel labeling approaches. These are compatible with super-resolution fluorescence microscopy to examine peptidoglycan synthesis, hydrolysis, and the localization of the enzymes required for its biosynthesis (penicillin binding proteins (PBPs)). Synthesis of a cephalosporin-based fluorescent probe revealed a pattern of PBPs at the septum during division, supporting a model of dispersed peptidoglycan synthesis. Metabolic and hydroxylamine-based probes respectively enabled the synthesis of glycan strands and associated reducing termini of the peptidoglycan to be mapped. Foci and arcs of reducing termini appear as a result of both synthesis of glycan strands and glucosaminidase activity of the major peptidoglycan hydrolase, SagB. Our studies provide molecular level details of how essential peptidoglycan dynamics are controlled during growth and division.
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DOI：
10.1002/anie.201206749
发表时间：
2012-12-07
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Kuru E;Hughes HV;Brown PJ;Hall E;Tekkam S;Cava F;de Pedro MA;Brun YV;VanNieuwenhze MS
通讯作者：
VanNieuwenhze MS
影响因子：
3.6
作者：
Turner RD;Vollmer W;Foster SJ
通讯作者：
Foster SJ
影响因子：
6.7
作者：
Reed P;Atilano ML;Alves R;Hoiczyk E;Sher X;Reichmann NT;Pereira PM;Roemer T;Filipe SR;Pereira-Leal JB;Ligoxygakis P;Pinho MG
通讯作者：
Pinho MG
DOI：
10.1073/pnas.1004304107
发表时间：
2010-11-02
影响因子：
11.1
作者：
Atilano, Magda L.;Pereira, Pedro M.;Filipe, Sergio R.
通讯作者：
Filipe, Sergio R.
影响因子：
28.3
作者：
Reichmann, Nathalie T.;Tavares, Andreia C.;Pinho, Mariana G.
通讯作者：
Pinho, Mariana G.