Characterization of the recA gene of Vibrio anguillarum.
Characterization of the recA gene of Vibrio anguillarum.
复制标题
鳗弧菌recA基因的表征。
DOI:
10.1016/0378-1119(92)90442-r
复制
发表时间:
1992
期刊:
影响因子:
3.5
通讯作者:
Crosa,JH
中科院分区:
文献类型:
--
作者:
Tolmasky,ME;Gammie,AE;Crosa,JH
We have cloned and sequenced therecAgene from two strains, 775 and 531A, of the fish pathogen,Vibrio anguillarum. Although both strains showed different sensitivities to methyl methanesulfonate (MMS), therecAgenes were identical. In vitro expression of theV. anguillarum rec Agene produced a polypeptide of about 40 kDa, in agreement with the value obtained from the nucleotide sequence. We identified the transcription start point by primer extension. The promoter for therecAgene mapped to an SOS regulatory element. The presence of an SOS box suggests that a LexA-like mediated response system may exist inV. anguillarum. The deduced RecA amino acid sequence is highly homologous withEscherichia coliRecA and other RecA proteins. Domains important in RecA function are conserved. We provide a comparative analysis of the activities and features of RecA analogs from a variety of species. We observed that certain residues that could be important in protein conformation are conserved in RecA proteins across a diverse range of bacterial species.
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影响因子:
3.6
作者:
A. Gammie;J. H. Crosa
通讯作者:
J. H. Crosa
影响因子:
3.5
作者:
Rajkumar Ramesar;V. Abratt;David R. Woods;D E Rawlings
通讯作者:
D E Rawlings
DOI:
10.1101/sqb.1984.049.01.057
发表时间:
1984
期刊:
Cold Spring Harbor symposia on quantitative biology
影响因子:
--
作者:
Blanar,MA;Kneller,D;Clark,AJ;Karu,AE;Cohen,FE;Langridge,R;Kuntz,ID
通讯作者:
Kuntz,ID
影响因子:
3.2
作者:
John T. Singer
通讯作者:
John T. Singer
影响因子:
3.1
作者:
CROSA, JH;HODGES, LL;SCHIEWE, MH
通讯作者:
SCHIEWE, MH