Characterization of the recA gene of Vibrio anguillarum.

Characterization of the recA gene of Vibrio anguillarum.
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鳗弧菌recA基因的表征。

DOI:
10.1016/0378-1119(92)90442-r
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发表时间:
1992
期刊:
影响因子:
3.5
通讯作者:
Crosa,JH
Crosa,JH
中科院分区:
生物学3区
文献类型:
--
作者:
Tolmasky,ME;Gammie,AE;Crosa,JH

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我们从鳗弧菌775和531A两个菌株中克隆了recA基因并进行了序列测定。虽然两株菌对甲磺酸甲酯(MMS)的敏感性不同,但recA基因是相同的。在体外表达的V. anguillarum rec Agene产生约40 kDa的多肽,与从核苷酸序列获得的值一致。我们通过引物延伸确定了转录起始点。therecA基因的启动子定位于SOS调控元件。SOS盒的存在表明V中可能存在LexA样介导的反应系统。鳗属推导的RecA氨基酸序列与大肠杆菌RecA及其它RecA蛋白高度同源。RecA功能中重要的结构域是保守的。我们提供了从各种物种的RecA类似物的活动和功能的比较分析。我们观察到,某些残基,可能是重要的蛋白质构象是保守的RecA蛋白在不同范围的细菌物种。
We have cloned and sequenced therecAgene from two strains, 775 and 531A, of the fish pathogen,Vibrio anguillarum. Although both strains showed different sensitivities to methyl methanesulfonate (MMS), therecAgenes were identical. In vitro expression of theV. anguillarum rec Agene produced a polypeptide of about 40 kDa, in agreement with the value obtained from the nucleotide sequence. We identified the transcription start point by primer extension. The promoter for therecAgene mapped to an SOS regulatory element. The presence of an SOS box suggests that a LexA-like mediated response system may exist inV. anguillarum. The deduced RecA amino acid sequence is highly homologous withEscherichia coliRecA and other RecA proteins. Domains important in RecA function are conserved. We provide a comparative analysis of the activities and features of RecA analogs from a variety of species. We observed that certain residues that could be important in protein conformation are conserved in RecA proteins across a diverse range of bacterial species.
DOI: 10.1111/j.1365-2958.1991.tb01861.x
发表时间: 1991
影响因子: 3.6
作者:
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通讯作者: J. H. Crosa
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发表时间: 1989
期刊: Gene
影响因子: 3.5
作者:
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发表时间: 1984
期刊: Cold Spring Harbor symposia on quantitative biology
影响因子: --
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DOI: 10.1128/jb.171.11.6367-6371.1989
发表时间: 1989
影响因子: 3.2
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通讯作者: John T. Singer
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发表时间: 1980-01-01
影响因子: 3.1
作者:
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