Transcriptional profiles underlying parent-of-origin effects in seeds of Arabidopsis thaliana.
Transcriptional profiles underlying parent-of-origin effects in seeds of Arabidopsis thaliana.
复制标题
拟南芥种子中产卵的父母效应的基础转录曲线。
DOI：
10.1186/1471-2229-10-72
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发表时间：
2010-04-20
影响因子：
5.3
通讯作者：
Scott RJ
中科院分区：
文献类型：
--
作者：
Tiwari S;Spielman M;Schulz R;Oakey RJ;Kelsey G;Salazar A;Zhang K;Pennell R;Scott RJ
同一物种但不同倍性的杂交植物可以对种子生长产生显著影响，但对对此负责的转录程序的改变知之甚少。亲本基因组不平衡特别影响胚乳的增殖，其中父源与母源贡献的基因组的比率增加（"父源过量"）与过度增殖相关，而母源过量抑制胚乳生长。一种解释是，倍性间杂交破坏了种子中亲本印记基因活性拷贝的平衡。这得到了以下观察结果的支持：拟南芥印记FIS类基因的突变与父本过量表型具有许多特征。在这里，我们研究了基因表达的起源的父母的影响，在拟南芥通过转录分析产生的角果倍性杂交和FIS类突变体。我们发现受精的fis1突变种子与父本过剩种子具有相似的特征，这表明共同的表型是由相似的基因表达模式所支撑的。我们确定了与增强或抑制种子生长密切相关的基因，这为进一步研究提供了许多候选基因，包括MADS盒转录因子、细胞周期基因和参与激素途径的基因。这里提出的工作是一个步骤，了解种子发育的父母的基因组平衡和印记的相关现象的影响。
Crossing plants of the same species but different ploidies can have dramatic effects on seed growth, but little is known about the alterations to transcriptional programmes responsible for this. Parental genomic imbalance particularly affects proliferation of the endosperm, with an increased ratio of paternally to maternally contributed genomes ('paternal excess') associated with overproliferation, while maternal excess inhibits endosperm growth. One interpretation is that interploidy crosses disrupt the balance in the seed of active copies of parentally imprinted genes. This is supported by the observation that mutations in imprinted FIS-class genes of Arabidopsis thaliana share many features of the paternal excess phenotype. Here we investigated gene expression underlying parent-of-origin effects in Arabidopsis through transcriptional profiling of siliques generated by interploidy crosses and FIS-class mutants. We found that fertilized fis1 mutant seeds have similar profiles to seeds with paternal excess, showing that the shared phenotypes are underpinned by similar patterns of gene expression. We identified genes strongly associated with enhanced or inhibited seed growth; this provided many candidates for further investigation including MADS-box transcription factors, cell cycle genes, and genes involved in hormone pathways. The work presented here is a step towards understanding the effects on seed development of the related phenomena of parental genome balance and imprinting.
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影响因子：
4.6
作者：
Chanvivattana, Y;Bishopp, A;Goodrich, J
通讯作者：
Goodrich, J
影响因子：
56.9
作者：
Grossniklaus, U;Vielle-Calzada, JP;Gagliano, WB
通讯作者：
Gagliano, WB
影响因子：
4.3
作者：
Baroux, C;Fransz, P;Grossniklaus, U
通讯作者：
Grossniklaus, U
影响因子：
11.6
作者：
Gutiérrez-Marcos, JF;Costa, LM;Dickinson, HG
通讯作者：
Dickinson, HG
影响因子：
14.9
作者：
Baumbusch, LO;Thorstensen, T;Aalen, RB
通讯作者：
Aalen, RB