Two-photon-induced fluorescence of isomorphic nucleobase analogs.
Two-photon-induced fluorescence of isomorphic nucleobase analogs.
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两光子诱导的异构核酶类似物的荧光。
DOI：
10.1002/cphc.201400031
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发表时间：
2014-04-04
期刊：
影响因子：
--
通讯作者：
Magennis SW
中科院分区：
文献类型：
--
作者：
Lane RS;Jones R;Sinkeldam RW;Tor Y;Magennis SW
五同构的荧光尿苷模拟物已进行双光子（2 P）激发分析，以探讨其潜在的适用性作为非微扰探针的单分子检测核酸。我们发现，小的结构差异，可以引起重大的变化，在双光子激发概率，与2 P截面变化超过一个数量级。两个探针，都是呋喃修饰的尿苷类似物，具有最高的2 P截面（3.8 GM和7.6 GM）报告的核碱基类似物，使用常规的钛：蓝宝石激光器在690 nm处激发，它们也具有最低的发射量子产率。相比之下，具有最高量子产率的类似物具有最低的2 P截面。这里提出的结构-物理性质关系是合理设计具有受控2 P特征的发射性核碱基类似物的第一步。结果表明，通过明智的结构修改的重大改进的潜力。
Five isomorphic fluorescent uridine mimics have been subjected to two-photon (2P) excitation analysis to investigate their potential applicability as non-perturbing probes for the single-molecule detection of nucleic acids. We find that small structural differences can cause major changes in the two-photon excitation probability, with the 2P cross sections varying by over one order of magnitude. Two of the probes, both furan-modified uridine analogs, have the highest 2P cross sections (3.8 GM and 7.6 GM) reported for nucleobase analogs, using a conventional Ti:sapphire laser for excitation at 690 nm; they also have the lowest emission quantum yields. In contrast, the analogs with the highest reported quantum yields have the lowest 2P cross sections. The structure-photophysical property relationship presented here is a first step towards the rational design of emissive nucleobase analogs with controlled 2P characteristics. The results demonstrate the potential for major improvements through judicious structural modifications.
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影响因子：
62.1
作者：
Sinkeldam, Renatus W.;Greco, Nicholas J.;Tor, Yitzhak
通讯作者：
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3.3
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2.4
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1.7
作者：
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