Detailed dissection of the chromosomal region containing the Ph1 locus in wheat Triticum aestivum: with deletion mutants and expression profiling.
Detailed dissection of the chromosomal region containing the Ph1 locus in wheat Triticum aestivum: with deletion mutants and expression profiling.
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DOI：
10.1093/aob/mcm252
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发表时间：
2008-04
期刊：
影响因子：
4.2
通讯作者：
Moore G
中科院分区：
文献类型：
--
作者：
Al-Kaff N;Knight E;Bertin I;Foote T;Hart N;Griffiths S;Moore G
小麦5 B染色体长臂上显性同源染色体配对抑制基因Ph 1的研究是本文研究的核心。Ph 1基因座限制染色体配对和重组在减数分裂真正的同源物。小麦作为一种作物的重要性和需要利用其野生亲属作为小麦育种计划中重要经济性状的供体，是揭示Ph 1基因座机制和调节其活性的主要驱动力。Ph 1基因座的分子遗传学表征之后，已经确定了覆盖该区域的五个额外的缺失突变体。此外，更多的细菌人工染色体（BAC）进行测序和分析，以阐明该位点的复杂性。用半定量RT-PCR方法比较了Ph 1基因5 B区与5A、5D区同源基因的表达谱。对PCR产物进行克隆和测序以鉴定其来源基因。缺失突变体和表达谱的基因在该地区含有Ph 1基因座5 B进一步限制Ph 1的cdk样基因簇。cdk样基因的生物信息学分析揭示了它们与来自人类的检查点激酶Cdk 2的密切同源性。cdk 2参与复制的起始，并且在早期减数分裂中是必需的。表达谱分析显示，cdk样基因簇在5 B上分析的区域内是独特的，因为这些基因被转录。5 B上cdk样基因座的缺失导致5A和5D上功能性cdk样拷贝的转录激活。因此，5 B上的cdk基因座在决定总体活性方面对5A和5D上的那些是显性的，这将取决于来自非功能性cdk样基因和功能性cdk样基因的转录之间的复杂相互作用。Ph 1基因座已被定义为与人类Cdk 2相关的cdk样基因簇，Cdk 2是参与复制起始并为早期减数分裂所需的主检查点基因。
Understanding Ph1, a dominant homoeologous chromosome pairing suppressor locus on the long arm of chromosome 5B in wheat Triticum aestivum L., is the core of the investigation in this article. The Ph1 locus restricts chromosome pairing and recombination at meiosis to true homologues. The importance of wheat as a crop and the need to exploit its wild relatives as donors for economically important traits in wheat breeding programmes is the main drive to uncover the mechanism of the Ph1 locus and regulate its activity. Following the molecular genetic characterization of the Ph1 locus, five additional deletion mutants covering the region have been identified. In addition, more bacterial artificial chromosomes (BACs) were sequenced and analysed to elucidate the complexity of this locus. A semi-quantitative RT–PCR was used to compare the expression profiles of different genes in the 5B region containing the Ph1 locus with their homoeologues on 5A and 5D. PCR products were cloned and sequenced to identify the gene from which they were derived. Deletion mutants and expression profiling of genes in the region containing the Ph1 locus on 5B has further restricted Ph1 to a cluster of cdk-like genes. Bioinformatic analysis of the cdk-like genes revealed their close homology to the checkpoint kinase Cdk2 from humans. Cdk2 is involved in the initiation of replication and is required in early meiosis. Expression profiling has revealed that the cdk-like gene cluster is unique within the region analysed on 5B in that these genes are transcribed. Deletion of the cdk-like locus on 5B results in activation of transcription of functional cdk-like copies on 5A and 5D. Thus the cdk locus on 5B is dominant to those on 5A and 5D in determining the overall activity, which will be dependent on a complex interplay between transcription from non-functional and functional cdk-like genes. The Ph1 locus has been defined to a cdk-like gene cluster related to Cdk2 in humans, a master checkpoint gene involved in the initiation of replication and required for early meiosis.
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影响因子：
2.6
作者：
Maestra, B;de Jong, JH;Naranjo, T
通讯作者：
Naranjo, T
影响因子：
7.2
作者：
Martinez-Perez, E;Shaw, P;Moore, G
通讯作者：
Moore, G
影响因子：
64.8
作者：
RILEY, R;CHAPMAN, V
通讯作者：
CHAPMAN, V
DOI：
10.1007/bf02906837
发表时间：
1986-01-01
期刊：
CARLSBERG RESEARCH COMMUNICATIONS
影响因子：
--
作者：
HOLM, PB
通讯作者：
HOLM, PB
影响因子：
21.3
作者：
Prieto, P;Shaw, P;Moore, G
通讯作者：
Moore, G