Self-assembled low-molecular-weight gelator injectable microgel beads for delivery of bioactive agents.
Self-assembled low-molecular-weight gelator injectable microgel beads for delivery of bioactive agents.
复制标题
自组装的低分子量凝胶剂可注射的微凝胶珠，用于递送生物活性剂。
DOI：
10.1039/d0sc06296k
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发表时间：
2021-02-02
期刊：
影响因子：
8.4
通讯作者：
Smith DK
中科院分区：
文献类型：
--
作者：
Piras CC;Kay AG;Genever PG;Smith DK
本文报道了低分子量凝胶因子DBS-CONHNH_2和天然多糖海藻酸钙聚合物凝胶因子PG的复合自组装微凝胶珠的制备。由于自组装网络的脆弱性和难以保持任何强加的形状，基于LMWG的微凝胶制剂非常罕见。我们的混合珠粒含有互穿的LMWG和PG网络，通过乳液法获得，可制备直径在mm或μm范围内的可控尺寸的球形凝胶颗粒。基于LMWG/藻酸盐的微凝胶可以容易地制备，具有可重复的直径<1 μm（约10 μ m）。800 nm）。它们在室温下的水中稳定数月，并通过注射器注射存活。LMWG在冷却时的快速组装在帮助控制微凝胶珠的直径方面起着积极的作用。这些LMWG微珠保留了母体凝胶递送生物活性分子肝素的能力，并且在细胞培养基中这增强了人类间充质干细胞的生长。因此，这种微凝胶可能在组织修复中有未来的应用。这种制备LMWG微凝胶的方法是一种平台技术，可以潜在地应用于各种不同功能的LMWG，因此具有广泛的潜力。我们报告的微凝胶珠直径约。800纳米，基于低分子量胶凝剂和聚合物胶凝剂的互穿网络，并证明它们可用作肝素递送载体来增强干细胞生长。
We report the preparation of hybrid self-assembled microgel beads by combining the low molecular weight gelator (LMWG) DBS-CONHNH2 and the natural polysaccharide calcium alginate polymer gelator (PG). Microgel formulations based on LMWGs are extremely rare due to the fragility of the self-assembled networks and the difficulty of retaining any imposed shape. Our hybrid beads contain interpenetrated LMWG and PG networks, and are obtained by an emulsion method, allowing the preparation of spherical gel particles of controllable sizes with diameters in the mm or μm range. Microgels based on LMWG/alginate can be easily prepared with reproducible diameters <1 μm (ca. 800 nm). They are stable in water at room temperature for many months, and survive injection through a syringe. The rapid assembly of the LMWG on cooling plays an active role in helping control the diameter of the microgel beads. These LMWG microbeads retained the ability of the parent gel to deliver the bioactive molecule heparin, and in cell culture medium this enhanced the growth of human mesenchymal stem cells. Such microgels may therefore have future applications in tissue repair. This approach to fabricating LMWG microgels is a platform technology, which could potentially be applied to a variety of different functional LMWGs, and hence has wide-ranging potential. We report microgel beads with diameters of ca. 800 nm based on interpenetrating networks of a low-molecular-weight gelator and a polymer gelator, and demonstrate their use as heparin delivery vehicles to enhance stem cell growth.
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影响因子：
9.7
作者：
Kim, Mihye;Kim, Young Ha;Tae, Giyoong
通讯作者：
Tae, Giyoong
影响因子：
4.9
作者：
Howe, Edward J.;Okesola, Babatunde O.;Smith, David K.
通讯作者：
Smith, David K.
影响因子：
4.9
作者：
Knaack, Sven;Lode, Anja;Gelinsky, Michael
通讯作者：
Gelinsky, Michael
影响因子：
5.9
作者：
James, Sally;Fox, James;Afsari, Farinaz;Lee, Jennifer;Clough, Sally;Knight, Charlotte;Ashmore, James;Ashton, Peter;Preham, Olivier;Hoogduijn, Martin;Ponzoni, Raquel De Almeida Rocha;Hancock, Y.;Coles, Mark;Genever, Paul
通讯作者：
Genever, Paul
影响因子：
62.1
作者：
Du X;Zhou J;Shi J;Xu B
通讯作者：
Xu B