Micro-techniques for analysis of human adipose tissue fatty acid composition in dietary studies.
Micro-techniques for analysis of human adipose tissue fatty acid composition in dietary studies.
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饮食研究中分析人体脂肪组织脂肪酸组成的微技术。
DOI：
10.1016/j.numecd.2012.11.003
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Hodson L
中科院分区：
文献类型：
--
作者：
Hodson L
背景和目的脂肪组织（AT）脂肪酸（FA）组成被认为是膳食脂肪酸摄入量的金标准长期生物标志物。通常，这种测量是直接从通过大针活检或切口收集的样本中进行的。然而，随着人们对AT与健康的关系的兴趣日益浓厚，理想情况下，脂肪酸组成将与其他测量一起分析，如基因表达或组织学，在单个AT样品上。在这里，我们评估获得AT测量FA组成的替代方法，在某些情况下与其他测量相结合。方法与结果将不同方法获得的组织FA组成与大针活检或手术活检组织FA组成进行比较。通过小针微型活检（n= 10）、从RNA“脂质层”（在RNA提取过程中获得，2个位点，每个位点n= 6）或从准备用于组织学的冷冻切片组织（n= 10）收集的AT中，脂肪酸组成没有显著差异。我们还评估了血浆NEFA组成作为皮下AT替代标志物的有效性（n= 58-80）。NEFA血浆FAs与AT血浆FAs呈显著相关（P< 0.05）。结论在极少量的组织上测定AT的FA组成是可行的。此外，还可以测量经过RNA提取用于基因表达的AT样品的富含脂质的“副产品”上的FA组成。切片的组织学样本也适用。这为多学科合作提供了进一步的机会，可能导致更好地应用饮食生物标志物。
Background and aimsAdipose tissue (AT) fatty acid (FA) composition is considered to be the gold standard long-term biomarker of dietary fatty acid intake. Typically this measurement is made directly from samples collected via large-needle-biopsy or incision. However, with growing interest in the role of AT in relation to health, ideally the fatty acid composition would be analysed along with other measurements, such as gene expression or histology, on a single AT sample. Here we assess alternative ways of obtaining AT for measuring FA composition, in some cases in conjunction with other measurements.Methods and resultsThe FA composition of tissue obtained via different methods was compared to that of tissue collected via large-needle or surgical biopsy. Fatty acid composition was not significantly different in AT collected by small-needle mini-biopsy (n= 10), from an RNA ‘lipid layer’ (obtained during RNA extraction, 2 sites,n= 6 for each), or from cryosectioned tissue prepared for histology (n= 10). We also assessed the usefulness of the composition of plasma NEFA as a surrogate marker of subcutaneous AT (n= 58–80). Most FAs in plasma NEFA correlated strongly with those in AT (P< 0.05).ConclusionIt is feasible to measure the FA composition of AT on very small amounts of tissue. Additionally, it is possible to measure FA composition on the lipid rich ‘by-product’ of AT samples undergoing RNA extraction for gene expression. Samples sectioned for histology are also suitable. This provides further opportunities for multidisciplinary collaborations that may lead to a better application of dietary biomarkers.
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影响因子：
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作者：
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DOI：
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DOI：
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发表时间：
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影响因子：
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