Myogenic potential of chick limb bud mesenchyme in micromass culture.
Myogenic potential of chick limb bud mesenchyme in micromass culture.
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微团培养中鸡肢芽间充质的生肌潜力。
DOI：
10.1007/bf00211829
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Solursh,M
中科院分区：
文献类型：
--
作者：
Archer,CW;Langille,RM;Teran,MA;Solursh,M
在有利于肌生成的条件下，通过微团培养来评估18-25期鸡肢间充质的肌生成潜力，并以检测到的分化（肌肌球蛋白阳性）单核细胞的比例来测量。结果发现，类似的肌源性电位存在于整个肢体间充质阶段18和19之间，但这种潜力减半的阶段20。在第21阶段，近端间充质表现出显着比远端间充质更多的肌生成，但这种差异被废除的阶段22。此后，肌生成越来越受到限制，从远端间充质，而在更近端的地区的潜力并没有显着增加后，阶段23。当分析在培养的第1天和第4天分化的全部肢体成肌细胞之间的比率时，发现在第20和23阶段存在两个明显的峰。这些比值峰值的意义尚不清楚，但可能与这些阶段前成肌细胞的不同增殖潜力有关。
The myogenic potential of chick limb mesenchyme from stages 18–25 was assessed by micromass culture under conditions conductive to myogenesis, and was measured as the proportion of differentiated (muscle myosin-positive) mononucleated cells detected. It was found that similar myogenic potentials existed in mesenchyme from whole limbs between stages 18 and 19, but this potential was halved by stage 20. At stage 21, proximal mesenchyme showed significantly more myogenesis than distal mesenchyme, but this difference was abolished by stage 22. Thereafter, myogenesis was increasingly restricted from the distal mesenchyme, whilst the potential in more proximal regions did not significantly increase after stage 23. When the ratio between total limb myoblasts which differentiated on days 1 and 4 of culture was analysed, it was found that two distinct peaks existed at stages 20 and 23. The significance of these ratio peaks is unclear, but may be related to different proliferative potentials of the pre-myoblasts at these stages.
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DOI：
10.1016/0045-6039(82)90072-0
发表时间：
1982-01-01
期刊：
CELL DIFFERENTIATION
影响因子：
--
作者：
ARCHER, CW;ROONEY, P;WOLPERT, L
通讯作者：
WOLPERT, L
原位杂交揭示了鸡肢芽中 I 型和 II 型胶原蛋白 mRNA 的差异空间分布。
影响因子：
4.6
作者：
C. J. Devlin;Paul M. Brickell;E. R. Taylor;A. Hornbruch;R. Craig;Lewis Wolpert
通讯作者：
Lewis Wolpert
影响因子：
2.7
作者：
COTTRILL, CP;ARCHER, CW;WOLPERT, L
通讯作者：
WOLPERT, L
上皮对肢体软骨形成的影响涉及细胞外基质和细胞形状。
影响因子：
2.7
作者：
Zanetti,NC;Solursh,M
通讯作者：
Solursh,M
影响因子：
2.7
作者：
AHRENS, PB;SOLURSH, M;REITER, RS
通讯作者：
REITER, RS