Characterisation of terrestrial acidophilic archaeal ammonia oxidisers and their inhibition and stimulation by organic compounds.
Characterisation of terrestrial acidophilic archaeal ammonia oxidisers and their inhibition and stimulation by organic compounds.
复制标题
陆地嗜酸古菌氨氧化剂的表征及其有机化合物的抑制和刺激
DOI：
10.1111/1574-6941.12353
复制
发表时间：
2014-09
影响因子：
4.2
通讯作者：
Prosser JI
中科院分区：
文献类型：
--
作者：
Lehtovirta-Morley LE;Ge C;Ross J;Yao H;Nicol GW;Prosser JI
自养氨氧化由两种不同的微生物进行：氨氧化古菌（AOA）和氨氧化细菌（AOB）。AOA在许多土壤中的数量超过了它们的细菌同行，有时是几个数量级，但由于缺乏培养的分离物，对其生理学知之甚少。虽然一些AOA已经从土壤中培养，但在纯培养中，维也纳亚硝化球菌是唯一的陆生AOA，并且在实验室中生长需要丙酮酸。在这里，我们描述了两个嗜酸性的陆地AOA的纯培养和表征的分离，代表的Escheridatus属亚硝化菌和他们的有机酸的反应。有趣的是，尽管它们的亲缘关系很近，但这两种亚硝化菌菌株在生理特征上表现出差异，包括比生长速率、温度偏好以及在一定程度上对有机化合物的反应。与N. viennensis，这两个亚硝化菌分离物被丙酮酸盐抑制，但它们的生长产量在草酰乙酸的存在下增加。这项研究表明AOA物种内和不同AOA属之间的生理多样性。对有机化合物的不同偏好可能会影响土壤中氨氧化剂的有利定位以及陆地生态系统中氨氧化群落的结构。
Autotrophic ammonia oxidation is performed by two distinct groups of microorganisms: ammonia-oxidising archaea (AOA) and ammonia-oxidising bacteria (AOB). AOA outnumber their bacterial counterparts in many soils, at times by several orders of magnitude, but relatively little is known of their physiology due to the lack of cultivated isolates. Although a number of AOA have been cultivated from soil, Nitrososphaera viennensis was the sole terrestrial AOA in pure culture and requires pyruvate for growth in the laboratory. Here, we describe isolation in pure culture and characterisation of two acidophilic terrestrial AOA representing the Candidatus genus Nitrosotalea and their responses to organic acids. Interestingly, despite their close phylogenetic relatedness, the two Nitrosotalea strains exhibited differences in physiological features, including specific growth rate, temperature preference and to an extent, response to organic compounds. In contrast to N. viennensis, both Nitrosotalea isolates were inhibited by pyruvate but their growth yield increased in the presence of oxaloacetate. This study demonstrates physiological diversity within AOA species and between different AOA genera. Different preferences for organic compounds potentially influence the favoured localisation of ammonia oxidisers within the soil and the structure of ammonia-oxidising communities in terrestrial ecosystems.
登录
查看更多内容
DOI：
10.1073/pnas.1109000108
发表时间：
2011-12-27
影响因子：
11.1
作者：
Gubry-Rangin, Cecile;Hai, Brigitte;Nicol, Graeme W.
通讯作者：
Nicol, Graeme W.
影响因子：
4.4
作者：
Jung, Man-Young;Park, Soo-Je;Rhee, Sung-Keun
通讯作者：
Rhee, Sung-Keun
影响因子：
9.7
作者：
Dias, Armando Cavalcante Franco;Hoogwout, Eelco Frans;van Elsas, Jan Dirk
通讯作者：
van Elsas, Jan Dirk
DOI：
10.1073/pnas.0409727102
发表时间：
2005-02-15
影响因子：
11.1
作者：
Konstantinidis, KT;Tiedje, JM
通讯作者：
Tiedje, JM
影响因子：
4.1
作者：
Aston, John E.;Apel, William A.;Peyton, Brent M.
通讯作者：
Peyton, Brent M.