The influence of single bursts versus single spikes at excitatory dendrodendritic synapses.
The influence of single bursts versus single spikes at excitatory dendrodendritic synapses.
复制标题
DOI：
10.1111/j.1460-9568.2011.07978.x
复制
发表时间：
2012-02
期刊：
影响因子：
--
通讯作者：
Chen WR
中科院分区：
文献类型：
--
作者：
Masurkar AV;Chen WR
神经元活动的同步被认为可以增强信息处理。有很多证据支持啮齿动物嗅球肾小球的节律性爆裂外部簇状细胞（ETC）通过树突状细胞兴奋协调肾小球中间神经元和二尖瓣细胞的激活。然而，由于爆裂在轴树皮质突触有不同的意义，目前尚不清楚ETC爆裂是否赋予了一个特定的功能优势，在树树突触网络的初步穗。为了回答这个问题，我们调查的影响，单ETC突发和尖峰与在体外大鼠嗅球制备在不同的处理水平，通过钙成像的突触前ETC树突，双电记录的ETC-interneuron突触对，和多细胞钙成像的ETC诱导的人口活动。我们的研究结果支持单个ETC爆发，与单个尖峰相比，驱动强大的突触前钙信号，这反过来又与初始单突触尖峰驱动的树突兴奋性突触后电位的深刻扩展相关。这种扩展可以由突发的尖峰依赖或尖峰独立分量驱动。在群体水平上，爆发性兴奋比单个锋电位更广泛、更可靠。这进一步支持了ETC网络，部分原因是兴奋性树-树突触爆裂的功能优势，协调与体内气味处理相关的行为相关频率的同步活动。
The synchronization of neuronal activity is thought to enhance information processing. There is much evidence supporting rhythmically bursting external tufted cells (ETCs) of the rodent olfactory bulb glomeruli coordinating the activation of glomerular interneurons and mitral cells via dendrodendritic excitation. However, as bursting has variable significance at axodendritic cortical synapses, it is not clear if ETC bursting imparts a specific functional advantage over the preliminary spike in dendrodendritic synaptic networks. To answer this question, we investigated the influence of single ETC bursts and spikes with the in-vitro rat olfactory bulb preparation at different levels of processing, via calcium imaging of presynaptic ETC dendrites, dual electrical recording of ETC–interneuron synaptic pairs, and multicellular calcium imaging of ETC-induced population activity. Our findings supported single ETC bursts, vs. single spikes, driving robust presynaptic calcium signaling, which in turn was associated with profound extension of the initial monosynaptic spike-driven dendrodendritic excitatory postsynaptic potential. This extension could be driven by either the spike-dependent or spike-independent components of the burst. At the population level, burst-induced excitation was more widespread and reliable compared with single spikes. This further supports the ETC network, in part due to a functional advantage of bursting at excitatory dendrodendritic synapses, coordinating synchronous activity at behaviorally relevant frequencies related to odor processing in vivo.
登录
查看更多内容
影响因子：
16.2
作者：
Christie, JM;Bark, C;Westbrook, GL
通讯作者：
Westbrook, GL
DOI：
10.1523/jneurosci.2368-09.2009
发表时间：
2009-10-28
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Gire DH;Schoppa NE
通讯作者：
Schoppa NE
影响因子：
16.2
作者：
Isaacson, JS
通讯作者：
Isaacson, JS
影响因子：
2.5
作者：
Christie, JM;Westbrook, GL
通讯作者：
Westbrook, GL
影响因子：
1.7
作者：
Cadetti, L;Belluzzi, O
通讯作者：
Belluzzi, O