Ribavirin inhibits Zika virus (ZIKV) replication in vitro and suppresses viremia in ZIKV-infected STAT1-deficient mice.
Ribavirin inhibits Zika virus (ZIKV) replication in vitro and suppresses viremia in ZIKV-infected STAT1-deficient mice.
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DOI：
10.1016/j.antiviral.2017.08.007
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发表时间：
2017-10
影响因子：
7.6
通讯作者：
Kobayashi T
中科院分区：
文献类型：
--
作者：
Kamiyama N;Soma R;Hidano S;Watanabe K;Umekita H;Fukuda C;Noguchi K;Gendo Y;Ozaki T;Sonoda A;Sachi N;Runtuwene LR;Miura Y;Matsubara E;Tajima S;Takasaki T;Eshita Y;Kobayashi T
寨卡热是一种由寨卡病毒（ZIKV）引起的蚊媒传染病，是一种流行病，目前尚无有效的治疗方法。最近在巴西和法属波利尼西亚爆发的ZIKV与胎儿小头畸形和其他疾病如格林-巴利综合征的发病率显著增加有关。由于目前没有特异性疗法或疫苗，因此早期开发预防ZIKV扩增的方法是高度优先的。为了验证常用的抗病毒药物，我们评估了利巴韦林（一种用于与干扰素-β（IFN-β）一起治疗丙型肝炎的药物）对ZIKV复制的影响。在哺乳动物细胞中，我们观察到病毒唑对ZIKV复制和ZIKV诱导的细胞死亡具有抑制作用，但没有细胞毒性作用。此外，我们发现缺乏I型IFN信号传导的STAT 1缺陷型小鼠对ZIKV感染高度敏感，并表现出致命的结果。利巴韦林消除ZIKV感染的STAT-1缺陷小鼠中的病毒血症。这些数据表明，抑制病毒RNA依赖性RNA聚合酶可能对治疗ZIKV感染有效。我们的数据为抑制ZIKV复制和预防寨卡热的机制提供了新的见解。利巴韦林抑制哺乳动物细胞中的ZIKV复制。利巴韦林防止ZIKV诱导的细胞凋亡和细胞死亡。利巴韦林给药消除ZIKV感染的STAT 1缺陷小鼠中的病毒血症。从而延长了存活时间。
Zika fever, a mosquito-borne infectious disease caused by Zika virus (ZIKV), is an epidemic disease for which no effective therapy has been established. The recent outbreaks of ZIKV in Brazil and French Polynesia have been linked to a considerable increase in the incidence of fetal microcephaly and other diseases such as Guillain-Barre syndrome. Because there is currently no specific therapy or vaccine, the early exploitation of a method to prevent expansion of ZIKV is a high priority. To validate commonly used antiviral drugs, we evaluated the effect of ribavirin, a drug used to treat hepatitis C with interferon-β (IFN-β), on ZIKV replication. In mammalian cells, we observed an inhibitory effect of ribavirin on ZIKV replication and ZIKV-induced cell death without cytotoxic effect. Furthermore, we found that STAT1-deficient mice, which lack type I IFN signaling, were highly sensitive to ZIKV infection and exhibited lethal outcome. Ribavirin abrogated viremia in ZIKV-infected STAT-1-deficient mice. These data suggest that the inhibition of viral RNA-dependent RNA polymerases may be effective for treatment of ZIKV infection. Our data provide a new insight into the mechanisms for inhibition of ZIKV replication and prevention of Zika fever. Ribavirin inhibits ZIKV replication in mammalian cells. Ribavirin prevents ZIKV-induced apoptosis and cell death. Ribavirin administration abrogates viremia in ZIKV-infected STAT1-deficient mice. Leading to a prolonged survival.
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DOI：
10.1126/science.aaf5036
发表时间：
2016-04-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Faria NR;Azevedo RDSDS;Kraemer MUG;Souza R;Cunha MS;Hill SC;Thézé J;Bonsall MB;Bowden TA;Rissanen I;Rocco IM;Nogueira JS;Maeda AY;Vasami FGDS;Macedo FLL;Suzuki A;Rodrigues SG;Cruz ACR;Nunes BT;Medeiros DBA;Rodrigues DSG;Queiroz ALN;da Silva EVP;Henriques DF;da Rosa EST;de Oliveira CS;Martins LC;Vasconcelos HB;Casseb LMN;Simith DB;Messina JP;Abade L;Lourenço J;Alcantara LCJ;de Lima MM;Giovanetti M;Hay SI;de Oliveira RS;Lemos PDS;de Oliveira LF;de Lima CPS;da Silva SP;de Vasconcelos JM;Franco L;Cardoso JF;Vianez-Júnior JLDSG;Mir D;Bello G;Delatorre E;Khan K;Creatore M;Coelho GE;de Oliveira WK;Tesh R;Pybus OG;Nunes MRT;Vasconcelos PFC
通讯作者：
Vasconcelos PFC
DOI：
10.1056/nejmoa1602412
发表时间：
2016-12-15
期刊：
The New England journal of medicine
影响因子：
--
作者：
Brasil P;Pereira JP Jr;Moreira ME;Ribeiro Nogueira RM;Damasceno L;Wakimoto M;Rabello RS;Valderramos SG;Halai UA;Salles TS;Zin AA;Horovitz D;Daltro P;Boechat M;Raja Gabaglia C;Carvalho de Sequeira P;Pilotto JH;Medialdea-Carrera R;Cotrim da Cunha D;Abreu de Carvalho LM;Pone M;Machado Siqueira A;Calvet GA;Rodrigues Baião AE;Neves ES;Nassar de Carvalho PR;Hasue RH;Marschik PB;Einspieler C;Janzen C;Cherry JD;Bispo de Filippis AM;Nielsen-Saines K
通讯作者：
Nielsen-Saines K
影响因子：
8.8
作者：
Faye, Oumar;Faye, Ousmane;Sall, Amadou Alpha
通讯作者：
Sall, Amadou Alpha
影响因子：
4.8
作者：
Bougie, I;Bisaillon, M
通讯作者：
Bisaillon, M
影响因子：
5.2
作者：
Abdel-Hady, M.;Bansal, S.;Kelly, D. A.
通讯作者：
Kelly, D. A.