White pupae phenotype of tephritids is caused by parallel mutations of a MFS transporter.
White pupae phenotype of tephritids is caused by parallel mutations of a MFS transporter.
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DOI：
10.1038/s41467-020-20680-5
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发表时间：
2021-01-21
影响因子：
16.6
通讯作者：
Schetelig MF
中科院分区：
文献类型：
--
作者：
Ward CM;Aumann RA;Whitehead MA;Nikolouli K;Leveque G;Gouvi G;Fung E;Reiling SJ;Djambazian H;Hughes MA;Whiteford S;Caceres-Barrios C;Nguyen TNM;Choo A;Crisp P;Sim SB;Geib SM;Marec F;Häcker I;Ragoussis J;Darby AC;Bourtzis K;Baxter SW;Schetelig MF
自20世纪50年代以来，在不育昆虫技术计划的框架内，大量释放不育的雄性昆虫有助于抑制害虫种群。在主要的园艺害虫--桔小实蝇、头蜡虫和葫芦丝夜蛾中，一种关键的表型白蛹(Wp)几十年来一直被用来选择性地在释放前移除雌虫，但其相关基因尚不清楚。在这里，我们使用经典和现代遗传学方法来鉴定和功能表征这些远缘关系果蝇物种的因果wp−突变。我们发现wp表型是由单个保守基因中的平行突变产生的。CRISPR/Cas9介导的wp基因敲除导致白蛾和菜蛾白蛹品系的快速产生。Wp−突变的保守表型和独立性质表明，该技术可以为生产其他主要医疗和农业害虫的性别鉴定菌株提供一种通用方法。几十年来，白蛹(Wp)表型一直被用来在遗传性别鉴定中选择性地去除雌蜂，但决定基因还不清楚。在这里，作者表明wp表型是由包含多个物种的基因的主要促进器超家族结构域的平行突变产生的。
Mass releases of sterilized male insects, in the frame of sterile insect technique programs, have helped suppress insect pest populations since the 1950s. In the major horticultural pests Bactrocera dorsalis, Ceratitis capitata, and Zeugodacus cucurbitae, a key phenotype white pupae (wp) has been used for decades to selectively remove females before releases, yet the gene responsible remained unknown. Here, we use classical and modern genetic approaches to identify and functionally characterize causal wp− mutations in these distantly related fruit fly species. We find that the wp phenotype is produced by parallel mutations in a single, conserved gene. CRISPR/Cas9-mediated knockout of the wp gene leads to the rapid generation of white pupae strains in C. capitata and B. tryoni. The conserved phenotype and independent nature of wp− mutations suggest this technique can provide a generic approach to produce sexing strains in other major medical and agricultural insect pests. The white pupae (wp) phenotype has been used for decades to selectively remove females of tephritid species in genetic sexing, but the determining gene is unknown. Here, the authors show that wp phenotype is produced by parallel mutations in a Major Facilitator Superfamily domain containing gene across multiple species.
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影响因子：
3.8
作者：
Aumann, Roswitha A.;Schetelig, Marc F.;Haecker, Irina
通讯作者：
Haecker, Irina
影响因子：
14.9
作者：
Kotov, Alexei A.;Adashev, Vladimir E.;Olenina, Ludmila V.
通讯作者：
Olenina, Ludmila V.
CRISPR-CAS9介导的基因失活的高度活性SGRNA的合理设计。
影响因子：
46.9
作者：
Doench, John G.;Hartenian, Ella;Graham, Daniel B.;Tothova, Zuzana;Hegde, Mudra;Smith, Ian;Sullender, Meagan;Ebert, Benjamin L.;Xavier, Ramnik J.;Root, David E.
通讯作者：
Root, David E.
DOI：
10.1016/0305-0491(91)90198-m
发表时间：
1991-01-01
影响因子：
2.2
作者：
BOURTZIS, K;PSACHOULIA, C;MARMARAS, VJ
通讯作者：
MARMARAS, VJ
DOI：
10.1126/science.aaa2850
发表时间：
2015-06-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hall AB;Basu S;Jiang X;Qi Y;Timoshevskiy VA;Biedler JK;Sharakhova MV;Elahi R;Anderson MA;Chen XG;Sharakhov IV;Adelman ZN;Tu Z
通讯作者：
Tu Z