Whole mouse blood microRNA as biomarkers for exposure to γ-rays and (56)Fe ion.
Whole mouse blood microRNA as biomarkers for exposure to γ-rays and (56)Fe ion.
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DOI：
10.3109/09553002.2010.549537
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发表时间：
2011-07
影响因子：
2.6
通讯作者：
Smilenov LB
中科院分区：
文献类型：
--
作者：
Templin T;Amundson SA;Brenner DJ;Smilenov LB
电离辐射暴露的生物标志物在各种情况下都很有用，例如医疗诊断成像、职业暴露和航天飞行。本研究探讨了小鼠外周血中microRNA （miRNA）表达特征在多大程度上可以用作低和高线性能量转移辐射暴露的生物标志物。小鼠分别接受0.5、1.5、5.0 Gy γ射线（剂量率为0.0136 Gy/s）和0.1、0.5 Gy 56Fe离子（剂量率为0.00208 Gy/s）辐照。辐照后6 h或24 h从全血中分离总RNA。每个辐照条件下使用3只动物。采用实时定量聚合酶链反应测定差异表达的miRNA。miRNA表达特征具有辐射类型特异性和剂量和时间依赖性。差异表达的miRNA在一种情况下（71%）或多种情况下（29%）表达。基于差异表达miRNA的分类器预测辐射类型或剂量的准确率在75%至100%之间。基因本体论分析表明，辐照诱导的miRNA参与了mRNA转录调控、核酸代谢和发育等多种生物过程的调控。电离辐射在小鼠血液中诱导的miRNA特征具有辐射类型和辐射剂量特异性。这些发现强调了辐射反应的复杂性和miRNA在其中的重要性。
Biomarkers of ionising radiation exposure are useful in a variety of scenarios, such as medical diagnostic imaging, occupational exposures, and spaceflight. This study investigates to what extent microRNA (miRNA) expression signatures in mouse peripheral blood can be used as biomarkers for exposures to radiation with low and high linear energy transfers. Mice were irradiated with doses of 0.5, 1.5, or 5.0 Gy γ-rays (dose rate of 0.0136 Gy/s) or with doses of 0.1 or 0.5 Gy 56Fe ions (dose rate of 0.00208 Gy/s). Total RNA was isolated from whole blood at 6 h or 24 h after irradiation. Three animals per irradiation condition were used. Differentially expressed miRNA were determined by means of quantitative real-time polymerase chain reaction. miRNA expression signatures were radiation type-specific and dose- and time-dependent. The differentially expressed miRNA were expressed in either one condition (71%) or multiple conditions (29%). Classifiers based on the differentially expressed miRNA predicted radiation type or dose with accuracies between 75% and 100%. Gene-ontology analyses show that miRNA induced by irradiation are involved in the control of several biological processes, such as mRNA transcription regulation, nucleic-acid metabolism, and development. miRNA signatures induced by ionising radiation in mouse blood are radiation type- and radiation dose-specific. These findings underline the complexity of the radiation response and the importance of miRNA in it.
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影响因子：
3.7
作者：
Meadows, Sarah K.;Dressman, Holly K.;Muramoto, Garrett G.;Himburg, Heather;Salter, Alice;Wei, ZhengZheng;Ginsburg, Geoff;Chao, Nelson J.;Nevins, Joseph R.;Chute, John P.
通讯作者：
Chute, John P.
影响因子：
2.8
作者：
Josson, Sajni;Sung, Shian-Ying;Lao, Kaiqin;Chung, Leland W. K.;Johnstone, Peter A. S.
通讯作者：
Johnstone, Peter A. S.
影响因子：
3.4
作者：
Fachin, Ana L.;Mello, Stephano S.;Sakamoto-Hojo, Elza T.
通讯作者：
Sakamoto-Hojo, Elza T.
影响因子：
3.4
作者：
Chaudhry, M. Ahmad
通讯作者：
Chaudhry, M. Ahmad
影响因子：
3.7
作者：
Hunter, Melissa Piper;Ismail, Noura;Zhang, Xiaoli;Aguda, Baltazar D.;Lee, Eun Joo;Yu, Lianbo;Xiao, Tao;Schafer, Jeffrey;Lee, Mei-Ling Ting;Schmittgen, Thomas D.;Nana-Sinkam, S. Patrick;Jarjoura, David;Marsh, Clay B.
通讯作者：
Marsh, Clay B.