Calibration Technique for Suppressing Residual Etalon Artifacts in Slit-Averaged Raman Spectroscopy.
Calibration Technique for Suppressing Residual Etalon Artifacts in Slit-Averaged Raman Spectroscopy.
复制标题
用于抑制缝隙平均拉曼光谱法中残留的Etalon伪像的校准技术。
DOI：
10.1177/00037028211046643
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发表时间：
2022-03
影响因子：
3.5
通讯作者：
Berger AJ
中科院分区：
文献类型：
--
作者：
Massie C;Chen K;Berger AJ
背照式电荷耦合器件(BI-CCD)阵列提高了量子效率，但也放大了拉丁效应，这是一种倍增的、依赖于波长的固定图案效应。当来自数百个BI-CCD行的光谱数据组合在一起时，平均光谱通常看起来不含标准具。这可以在行级屏蔽实质上的调光，即使已经对BI-CCD进行了处理以抑制该效果。本说明比较了标准具校正的两种方法，一种是简单平均，另一种是使用荧光标准逐行校正。两个BI-CCD阵列被用来获取小鼠骨骼标本的拉曼光谱，这两个阵列都是由供应商为减少拉曼反应而粗化的。对于一个阵列，在所选择的曝光条件下，调色剂是主要的噪声源，即使对于所有行的平均光谱也是如此；近红外激发的拉曼峰受到显著影响。在这种情况下，逐行校准提高了平均光谱的光谱质量。另一台CCD的性能受到散粒噪声的限制，因此没有从额外的校准中获得任何好处。不同的结果强调了在存在大的荧光背景的情况下测量微弱的拉曼信号时检查和校正行级固定图案的重要性。
Back-illuminated charged-coupled device (BI-CCD) arrays increase quantum efficiency but also amplify etaloning, a multiplicative, wavelength-dependent fixed-pattern effect. When spectral data from hundreds of BI-CCD rows are combined, the averaged spectrum will generally appear etalon-free. This can mask substantial etaloning at the row level, even if the BI-CCD has been treated to suppress the effect. This Note compares two methods of etalon correction, one with simple averaging and one with row-by-row calibration using a fluorescence standard. Two BI-CCD arrays, both roughened by the supplier to reduce etaloning, were used to acquire Raman spectra of murine bone specimens. For one array, etaloning was the dominant source of noise under the exposure conditions chosen, even for the averaged spectrum across all rows; near-infrared-excited Raman peaks were noticeably affected. In this case, row-by-row calibration improved the spectral quality of the average spectrum. The other CCD’s performance was shot-noise limited and therefore received no benefit from the extra calibration. The different results highlight the importance of checking for and correcting row-level fixed pattern when measuring weak Raman signals in the presence of a large fluorescence background.
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影响因子：
7.4
作者：
Auguie, Baptiste;Reigue, Antoine;Etchegoin, Pablo G.
通讯作者：
Etchegoin, Pablo G.
影响因子：
7.4
作者：
SAVITZKY, A;GOLAY, MJE
通讯作者：
GOLAY, MJE
DOI：
10.3964/j.issn.1000-0593(2014)07-1995-05
发表时间：
2014-07-01
影响因子：
0.7
作者：
Ma Liang;Wei Jun;Huang Xiao-xian
通讯作者：
Huang Xiao-xian
影响因子：
2.5
作者：
Zhang, Hong;Li, Xiaogang;Huang, Yizhong
通讯作者：
Huang, Yizhong
影响因子：
3.5
作者：
Cooper, John B.;Abdelkader, Mohamed;Wise, Kent L.
通讯作者：
Wise, Kent L.