Particulate matter promotes in vitro receptor-recognizable low-density lipoprotein oxidation and dysfunction of lipid receptors.
Particulate matter promotes in vitro receptor-recognizable low-density lipoprotein oxidation and dysfunction of lipid receptors.
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DOI：
10.1002/jbt.21452
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发表时间：
2013-01
影响因子：
3.6
通讯作者：
Osornio-Vargas, Alvaro R.
中科院分区：
文献类型：
--
作者：
Manzano-Leon, Natalia;Mas-Oliva, Jaime;Sevilla-Tapia, Laura;Morales-Barcenas, Rocio;Serrano, Jesus;O'Neill, Marie S.;Garcia-Cuellar, Claudia M.;Quintana, Raul;Vazquez-Lopez, Ines;Osornio-Vargas, Alvaro R.
颗粒物可能会促进心血管疾病，这可能是其氧化潜能的结果。对易感动物的研究表明，颗粒物质通过增加斑块中的脂质/巨噬细胞含量而加重动脉粥样硬化。巨噬细胞脂质摄取需要氧化的低密度脂蛋白和清道夫受体；同样的受体也参与颗粒物质的摄取。我们研究了体外颗粒物氧化低密度脂蛋白和随后通过清道夫受体的细胞摄取的潜力。采用硫代巴比妥酸法评价颗粒物质诱导的低密度脂蛋白氧化。用荧光标记的低密度脂蛋白检测野生型和清道夫受体转染的中国仓鼠卵巢细胞和RAW264.7细胞的结合/内化。剂量依赖性结合/内化仅发生在清道夫受体转染的中国仓鼠卵巢细胞和RAW264.7细胞中。利用颗粒的竞争结合/内化表明，颗粒物质诱导颗粒氧化低密度脂蛋白和天然低密度脂蛋白的结合（~50%）和内化（~70%）减少。结果表明，颗粒物能够氧化低密度脂蛋白，有利于巨噬细胞内化，也改变了清道夫和低密度脂蛋白受体的功能。
Particulate matter may promote cardiovascular disease, possibly as a consequence of its oxidative potential. Studies using susceptible animals indicate that particulate matter aggravates atherosclerosis by increasing lipid/macrophage content in plaques. Macrophage lipid uptake requires oxidized low-density lipoprotein and scavenger receptors; same receptors are involved in particulate matter uptake. We studied in vitro particulate matter potential to oxidize low-density lipoproteins and subsequent cell uptake through scavenger receptors. Particulate matter-induced low-density lipoproteins oxidation was evaluated by the thiobarbituric acid assay. Binding/internalization was tested in wild type and scavenger receptor–transfected Chinese hamster ovary cells, and in RAW264.7 cells using fluorescently labeled low-density lipoproteins. Dose-dependent binding/internalization only occurred in scavenger receptor–transfected Chinese hamster ovary cells and RAW264.7 cells. Competition binding/internalization using particles showed that particulate matter induced decreased binding (~50%) and internalization (~70%) of particle-oxidized low-density lipoproteins and native low-density lipoproteins. Results indicate that particulate matter was capable of oxidizing low-density lipoproteins, favoring macrophage internalization, and also altered scavenger and low-density lipoproteins receptor function.
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影响因子：
2.1
作者：
Knaapen, AM;Schins, RPF;Borm, PJA
通讯作者：
Borm, PJA
影响因子：
2.1
作者：
Monn, C;Fendt, R;Koller, T
通讯作者：
Koller, T
DOI：
10.1080/00984100290071658
发表时间：
2002-10-01
期刊：
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A
影响因子：
--
作者：
Oberdörster, G;Sharp, Z;Cox, C
通讯作者：
Cox, C
影响因子：
37.8
作者：
Magari, SR;Hauser, R;Christiani, DC
通讯作者：
Christiani, DC
DOI：
10.1016/j.bbrc.2006.10.077
发表时间：
2006-12-22
影响因子：
3.1
作者：
Manzano-Leon, Natalia;Delgado-Coello, Blanca;Mas-Oliva, Jaime
通讯作者：
Mas-Oliva, Jaime