A cost-effective barcode system for maize genetic discrimination based on bi-allelic InDel markers
A cost-effective barcode system for maize genetic discrimination based on bi-allelic InDel markers
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基于双等位基因 InDel 标记的经济高效的玉米遗传判别条形码系统
DOI：
10.1186/s13007-020-00644-y
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发表时间：
2020-07
期刊：
影响因子：
5.1
通讯作者：
Han Zhao
中科院分区：
文献类型：
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作者：
Shuaiqiang Liang;Feng Lin;Yiliang Qian;Tifu Zhang;Yibo Wu;Yaocheng Qi;Sihai Ren;Long Ruan;Han Zhao
玉米是世界上最重要的谷类作物之一，拥有约2.3亿酶的复杂基因组，在不同种质间表现出巨大的表型和分子多样性。随着表型鉴定，分子标记已被广泛接受作为一种替代工具来区分不同的基因型。结果利用以往205个玉米品系的重测序数据，筛选了玉米全基因组的双等位基因插入缺失（InDels），构建了由37个多态信息含量（PIC）高、品种间判别大小大的双等位基因插入缺失标记组成的条形码系统。对条形码系统进行了测量和测定，利用这些标记对不同的玉米杂交种和自交系进行了清晰有效的区分，准确地确定了对应亲本的杂交种。通过与200多个玉米系的芯片数据比较，该条形码系统能够以1.57%的不同位点作为阈值来区分玉米品种。该条码系统可以标准化、简单、快速地使用，成本极低，对设备的要求最低。结论构建了一个用于玉米品系遗传鉴定的条形码系统，包含37个PIC值较高且使用方便的InDel标记。测试了该条形码系统对玉米品系的有效识别。
BackgroundMaize is one of the most important cereal crop all over the world with a complex genome of about 2.3 gigabase, and exhibits tremendous phenotypic and molecular diversity among different germplasms. Along with the phenotype identification, molecular markers have been accepted extensively as an alternative tool to discriminate different genotypes.ResultsBy using previous re-sequencing data of 205 lines, bi-allelic insertions and deletions (InDels) all over maize genome were screened, and a barcode system was constructed consisting of 37 bi-allelic insertion-deletion markers with high polymorphism information content (PIC) values, large discriminative size among varieties. The barcode system was measured and determined, different maize hybrids and inbreds were clearly discriminated efficiently with these markers, and hybrids responding parents were accurately determined. Compared with microarray data of more than 200 maize lines, the barcode system can discriminate maize varieties with 1.57% of different loci as a threshold. The barcode system can be used in standardized easy and quick operation with very low cost and minimum equipment requirements.ConclusionA barcode system was constructed for genetic discrimination of maize lines, including 37 InDel markers with high PIC values and user-friendly. The barcode system was measured and determined for efficient identification of maize lines.
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影响因子：
4.4
作者：
Zhang T;Gu M;Liu Y;Lv Y;Zhou L;Lu H;Liang S;Bao H;Zhao H
通讯作者：
Zhao H
影响因子：
10
作者：
Li, Xiwen;Yang, Yang;Chen, Shilin
通讯作者：
Chen, Shilin
DOI：
10.4238/2014.april.29.17
发表时间：
2014-04
期刊：
Genetics and molecular research : GMR
影响因子：
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作者：
X. Y. Li;H. X. Xu;J. W. Chen
通讯作者：
X. Y. Li;H. X. Xu;J. W. Chen
影响因子：
5.6
作者：
Sohn HB;Kim SJ;Hwang TY;Park HM;Lee YY;Markkandan K;Lee D;Lee S;Hong SY;Song YH;Koo BC;Kim YH
通讯作者：
Kim YH
影响因子：
5.3
作者：
通讯作者：
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