Melanocytes in black-boned chicken have immune contribution under infectious bursal disease virus infection.
Melanocytes in black-boned chicken have immune contribution under infectious bursal disease virus infection.
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乌鸡黑素细胞在传染性法氏囊病病毒感染下具有免疫贡献
DOI：
10.1016/j.psj.2021.101498
复制
发表时间：
2021-12
期刊：
影响因子：
4.4
通讯作者：
Deng X
中科院分区：
文献类型：
--
作者：
Han D;Tai Y;Hua G;Yang X;Chen J;Li J;Deng X
乌骨鸡的内脏器官中广泛分布着黑素细胞。然而，这些细胞的作用还没有完全阐明。本研究以3周龄雌性丝羽乌骨鸡为研究对象，探讨了传染性法氏囊病病毒（IBDV）感染对黑素细胞功能的影响。结果发现，感染IBDV的法氏囊内黑素细胞大量转移到结节和固有层，并有明显的凋亡细胞和BAX的高表达。定量PCR检测到与Toll样受体（TLR）信号通路相关的基因，包括TLR 1、TLR 3、TLR 4、TLR 15、髓样分化蛋白-88、干扰素-α和干扰素-β。我们随后分离并在体外用IBDV感染原代黑素细胞，发现在感染后24和48 h检测到较高的免疫基因表达;上调的先天性和获得性免疫基因参与了IBDV感染的发病机制，包括TLR 3、TLR 7、白细胞介素15（IL 15）、IL 18、IL 1 rap、CD 7、BG 2、ERAP 1和SLA 2。这些基因表达的变化与基于微管的运动、抗原加工和呈递、对病毒的防御和先天免疫反应高度相关。结果表明，乌骨鸡体内广泛分布的黑素细胞在病毒感染过程中可以迁移，发挥重要的天然免疫功能。
In black-boned chicken, melanocytes are widely distributed in their inner organs. However, the roles of these cells are not fully elucidated. In this study, we used 3-wk-old female Silky Fowl to investigate the functions of melanocytes under infection with infectious bursal disease virus (IBDV). We found the melanocytes in the bursa of Fabricius involved in IBDV infection shown as abundant melanin were transported into the nodule and lamina propria where obvious apoptotic cells and higher expression of BAX were detected. Genes related to the toll-like receptor (TLR) signaling pathway were highly detected by quantitative PCR, including TLR1, TLR3, TLR4, TLR15, myeloid differential protein-88, interferon-α, and interferon-β. We then isolated and infected primary melanocytes with IBDV in vitro and found that higher expressions of immune genes were detected at 24 and 48 h after infection; the upregulated innate and adaptive immune genes were involved in the pathogenesis of IBDV infection, including TLR3, TLR7, interleukin 15 (IL15), IL18, IL1rap, CD7, BG2, ERAP1, and SLA2. These changes in gene expression were highly associated with microtubule-based movement, antigen processing and presentation, defense against viruses, and innate immune responses. Our results indicated that the widely distributed melanocytes in Silky Fowl could migrate to play important innate immune roles during virus infection.
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DOI：
10.1002/ar.20623
发表时间：
2008-01-01
影响因子：
2
作者：
Ortolani-Machado, Claudia;De Freitas, Patricia;Faraco, Cloris
通讯作者：
Faraco, Cloris
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DOI：
10.1073/pnas.1805474115
发表时间：
2018-10-16
影响因子：
11.1
作者：
Lin, Wenlong;Su, Fasheng;Wang, Xiaojian
通讯作者：
Wang, Xiaojian
DOI：
10.1034/j.1600-0749.2002.02065.x
发表时间：
2002-12-01
期刊：
PIGMENT CELL RESEARCH
影响因子：
--
作者：
Lu, Y;Zhu, WY;Gu, JX
通讯作者：
Gu, JX
影响因子：
3.7
作者：
Han D;Wang S;Hu Y;Zhang Y;Dong X;Yang Z;Wang J;Li J;Deng X
通讯作者：
Deng X
影响因子：
4.5
作者：
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通讯作者：
Andersson L