Hyperpigmentation Results in Aberrant Immune Development in Silky Fowl (Gallus gallus domesticus Brisson).
Hyperpigmentation Results in Aberrant Immune Development in Silky Fowl (Gallus gallus domesticus Brisson).
复制标题
DOI：
10.1371/journal.pone.0125686
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Deng X
中科院分区：
文献类型：
--
作者：
Han D;Wang S;Hu Y;Zhang Y;Dong X;Yang Z;Wang J;Li J;Deng X
乌骨鸡（SF）是一种黑素细胞表型特殊、分布不典型的禽类。虽然与黑素细胞迁移相关的基因已被大量研究，但关于黑素细胞在内脏器官中的出生后分布以及色素沉着过度对SF发展的影响的信息很少。在这里，我们分析了黑素细胞分布在26个组织或器官出生后第1天和第2，3，4，6，10和23周。除肝脏、胰腺、脑垂体和肾上腺外，黑素细胞分布于全身，主要分布在血管周围。观察到黑素细胞和组织细胞之间的相互作用，并且黑素通过丝状伪足递送通过吞噬和内化的膜包裹的黑素体进行运输。小于10周龄的SF的脾脏、胸腺和腔上囊指数低于白色来航鸡（WL）。SF组脾脏IFN-γ和IL-4基因表达水平及血清抗H5 N1和传染性法氏囊病病毒抗体水平均低于WL组。乌骨鸡与非乌骨鸡杂交F_2代免疫器官发育存在差异。但在性成熟后，胸腺和腔上囊的退化发生在SF比WL晚。凋亡细胞和凋亡相关的Bax和Bcl-2蛋白的分析表明，细胞凋亡参与了胸腺和法氏囊的退化。提示黑素细胞色素沉着可能与黑素细胞免疫发育密切相关，为研究黑素细胞的作用提供了重要的动物模型。
The Silky Fowl (SF) is known for its special phenotypes and atypical distribution of melanocytes among internal organs. Although the genes associated with melanocyte migration have been investigated substantially, there is little information on the postnatal distribution of melanocytes in inner organs and the effect of hyperpigmentation on the development of SF. Here, we analyzed melanocyte distribution in 26 tissues or organs on postnatal day 1 and weeks 2, 3, 4, 6, 10, and 23. Except for the liver, pancreas, pituitary gland, and adrenal gland, melanocytes were distributed throughout the body, primarily around blood vessels. Interaction between melanocytes and the tissue cells was observed, and melanin was transported by filopodia delivery through engulfed and internalized membrane-encapsulated melanosomes. SFs less than 10 weeks old have lower indices of spleen, thymus, and bursa of Fabricius than White Leghorns (WLs). The expression levels of interferon-γ and interlukin-4 genes in the spleen, and serum antibody levels against H5N1 and infectious bursal disease virus were lower in SF than in WL. We also found immune organ developmental difference between Black-boned and non-Black- boned chickens from SFs and WLs hybrid F2 population. However, degeneration of the thymus and bursa of Fabricius occurred later in SF than in WL after sexual maturity. Analysis of apoptotic cells and apoptosis-associated Bax and Bcl-2 proteins indicated that apoptosis is involved in degeneration of the thymus and bursa of Fabricius. Therefore, these results suggest that hyperpigmentation in SF may have a close relationship with immune development in SF, which can provide an important animal model to investigate the roles of melanocyte.
登录
查看更多内容
DOI：
10.1002/ar.20623
发表时间：
2008-01-01
影响因子：
2
作者：
Ortolani-Machado, Claudia;De Freitas, Patricia;Faraco, Cloris
通讯作者：
Faraco, Cloris
影响因子：
3.6
作者：
Plonka PM;Passeron T;Brenner M;Tobin DJ;Shibahara S;Thomas A;Slominski A;Kadekaro AL;Hershkovitz D;Peters E;Nordlund JJ;Abdel-Malek Z;Takeda K;Paus R;Ortonne JP;Hearing VJ;Schallreuter KU
通讯作者：
Schallreuter KU
影响因子：
1.2
作者：
Muroya, S;Tanabe, R;Chikuni, K
通讯作者：
Chikuni, K
影响因子：
2
作者：
Nishimura, S.;Oshima, I.;Iwamoto, H.
通讯作者：
Iwamoto, H.
影响因子：
2.8
作者：
Li, Yulin;Zhu, Xuping;Deng, Xuemei
通讯作者：
Deng, Xuemei