Inverse regulation of light harvesting and photoprotection is mediated by a 3'-end-derived sRNA in cyanobacteria.
Inverse regulation of light harvesting and photoprotection is mediated by a 3'-end-derived sRNA in cyanobacteria.
复制标题
蓝藻中 3′ 端衍生的 sRNA 介导光捕获和光保护的反向调节
DOI：
10.1093/plcell/koaa030
复制
发表时间：
2021-04-17
期刊：
影响因子：
--
通讯作者：
Kirilovsky D
中科院分区：
文献类型：
--
作者：
Zhan J;Steglich C;Scholz I;Hess WR;Kirilovsky D
摘要藻胆体是蓝藻的主要触角，是自然界中最有效的大分子结构之一，用于光捕获和向光合反应中心的定向能量转移。然而，在不利的条件下，过量的激发能量需要迅速消散，以避免光损伤。橙子类胡萝卜素蛋白（OCP）在胁迫条件下感知光强度并诱导热能耗散。因此，它的表达必须严格控制，然而，这种调节的分子机制仍有待阐明。在这里，我们描述了集胞藻属PCC 6803中的转录后调节机制的发现，其中编码PBS的别藻蓝蛋白亚基的操纵子的表达直接和以反向方式与OCP的表达相关联。这种调节由ApcZ介导，ApcZ是一种来自四顺反子apcABC-apcZ操纵子3′端的小调控RNA。ApcZ在无应激条件下抑制ocp翻译。在大多数胁迫条件下，apc操纵子转录减少，ocp翻译增加。因此，参与光能收集的关键操纵子在功能上与参与能量耗散的蛋白质的表达相关。我们的研究结果支持了细菌中调节RNA网络通过mRNA 3 '-UTR的功能化而进化的观点。
Abstract Phycobilisomes (PBSs), the principal cyanobacterial antenna, are among the most efficient macromolecular structures in nature, and are used for both light harvesting and directed energy transfer to the photosynthetic reaction center. However, under unfavorable conditions, excess excitation energy needs to be rapidly dissipated to avoid photodamage. The orange carotenoid protein (OCP) senses light intensity and induces thermal energy dissipation under stress conditions. Hence, its expression must be tightly controlled; however, the molecular mechanism of this regulation remains to be elucidated. Here, we describe the discovery of a posttranscriptional regulatory mechanism in Synechocystis sp. PCC 6803 in which the expression of the operon encoding the allophycocyanin subunits of the PBS is directly and in an inverse fashion linked to the expression of OCP. This regulation is mediated by ApcZ, a small regulatory RNA that is derived from the 3′-end of the tetracistronic apcABC–apcZ operon. ApcZ inhibits ocp translation under stress-free conditions. Under most stress conditions, apc operon transcription decreases and ocp translation increases. Thus, a key operon involved in the collection of light energy is functionally connected to the expression of a protein involved in energy dissipation. Our findings support the view that regulatory RNA networks in bacteria evolve through the functionalization of mRNA 3′-UTRs.
登录
查看更多内容
DOI：
10.1074/jbc.m112.391755
发表时间：
2012-09-28
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Eisenhut M;Georg J;Klähn S;Sakurai I;Mustila H;Zhang P;Hess WR;Aro EM
通讯作者：
Aro EM
影响因子：
6.4
作者：
Grabowicz M;Koren D;Silhavy TJ
通讯作者：
Silhavy TJ
影响因子：
4.3
作者：
Calzadilla, Pablo I.;Muzzopappa, Fernando;Kirilovsky, Diana
通讯作者：
Kirilovsky, Diana
DOI：
10.1073/pnas.0600927103
发表时间：
2006-05-02
影响因子：
11.1
作者：
Dühring, U;Axmann, IM;Wilde, A
通讯作者：
Wilde, A
影响因子：
4.3
作者：
Arteni, Ana A.;Ajlani, Ghada;Boekema, Egbert J.
通讯作者：
Boekema, Egbert J.