Reproducibility of creatine kinase reaction kinetics in human heart: a (31) P time-dependent saturation transfer spectroscopy study.
Reproducibility of creatine kinase reaction kinetics in human heart: a (31) P time-dependent saturation transfer spectroscopy study.
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DOI：
10.1002/nbm.3103
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发表时间：
2014-06
影响因子：
2.9
通讯作者：
Gropler, Robert
中科院分区：
文献类型：
--
作者：
Bashir, Adil;Gropler, Robert
关键词：
肌酸激酶（CK）对于心脏组织中三磷酸腺苷（ATP）的缓冲和快速再生至关重要。在这里，我们展示了一个31 P磁共振波谱（MRS）协议，以量化CK反应动力学在人类心肌在3 T。此外，我们试图量化测得的代谢参数的重测信度。该方法采用改进的1D图像选择在体光谱（ISIS）和时间依赖饱和转移（TDST）方法定位来自心脏的31 P信号，用于测量CK反应参数。测试了15名健康志愿者（共22次测量）。CK反应速率常数（kf）为0.32 ± 0.05 sec-1，变异系数（CV）= 15.62%。磷酸肌酸（PCr）的固有T1为7.36 ± 1.79秒，CV = 24.32%。这些数值与先前报告的数值一致。PCr/ATP比值为1.94 ± 0.15，CV = 7.73%，在健康受试者范围内。该技术的重复性进行了测试，在7个科目和推断参数，如kf和T1表现出良好的可靠性（组内相关系数（ICC）= 0.90和0.79的kf和T1分别）。本研究中提供的重现性数据将能够计算临床和研究所需的把握度和样本量。该技术将允许在临床和研究中检查心脏能量代谢，从而深入了解心脏能量不足和功能缺陷之间的关系。
Creatine Kinase (CK) is essential for buffering and rapid regeneration of adenosine triphosphate (ATP) in heart tissue. Herein we demonstrate a 31P magnetic resonance spectroscopy (MRS) protocol to quantify CK reaction kinetics in human myocardium at 3T. Furthermore we sought to quantify the test–retest reliability of the measured metabolic parameters. The method localizes 31P signal from heart using modified 1D image selected in vivo spectroscopy (ISIS) and a time dependent saturation transfer (TDST) approach was used to measure CK reaction parameters. Fifteen healthy volunteers (22 measurements total) were tested. The CK reaction rate constant (kf) was 0.32 ± 0.05 sec−1 and coefficient of variation (CV) = 15.62%. Intrinsic T1 for phosphocreatine (PCr) was 7.36 ± 1.79 sec with CV = 24.32%. These values are consistent those previously reported. PCr/ATP ratio was equal to 1.94 ± 0.15 with CV = 7.73%, which is within the range of healthy subjects. The reproducibility of the technique was tested in 7 subjects and inferred parameters such as kf and T1 exhibited good reliability (intraclass correlation coefficient (ICC) = 0.90 and 0.79 for kf and T1 respectively). The reproducibility data provided in this study will enable calculation of power and sample sizes required for clinical and research studies. The technique will allow for the examination of cardiac energy metabolism in clinical and research studies providing insight in the relationship between energy deficit and functional deficiency in heart.
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DOI：
10.1152/ajpheart.1995.269.3.h1030
发表时间：
1995-09-01
影响因子：
4.8
作者：
HAMMAN, BL;BITTL, JA;INGWALL, JS
通讯作者：
INGWALL, JS
影响因子：
14
作者：
Hudsmith, Lucy E.;Neubauer, Stefan
通讯作者：
Neubauer, Stefan
影响因子：
2.8
作者：
Lipskaya, TY
通讯作者：
Lipskaya, TY
影响因子：
3.3
作者：
Goudemant, JF;Francaux, M;Sturbois, X
通讯作者：
Sturbois, X
影响因子：
4.4
作者：
FORSEN, S;HOFFMAN, RA
通讯作者：
HOFFMAN, RA