Two monoclonal antibodies recognising aa 634-668 and aa 1026-1055 of NogoA enhance axon extension and branching in cultured neurons.
Two monoclonal antibodies recognising aa 634-668 and aa 1026-1055 of NogoA enhance axon extension and branching in cultured neurons.
复制标题
DOI：
10.1371/journal.pone.0088554
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Deng B;Gao F;Liu FF;Zhao XH;Yu CY;Ju G;Xu LX;Wang J
在以前的研究中，我们在小鼠中制备了两种单抗，aNogoA-N和aNogo-66 mAb，分别针对大鼠NogoA和Nogo-66的重组N端片段而制备。在免疫荧光组织化学(IHC)染色和免疫印迹(WB)分析中，与商品兔抗大鼠NogoA多克隆抗体(PAb)相比，这两种单抗对NogoA抗原也具有特异性。在大肠杆菌中表达了覆盖NogoA(AA 570-691)和NOGO-66(AA 1026-1091)N-末端的系列截短片段。ANogoA-N和aNogo-66识别的表位分别位于NogoA的AA634-668和AA1026-1055区域。两种单抗均能显著促进体外培养的海马神经元的轴突生长和分支。这些结果表明，与NogoA的AA634-668和AA1026-1055结合的抗体可能对轴突的生长和分支有刺激作用。此外，我们生成的两个mAb是针对Nogoa的，并且显著阻止了Nogoa的功能。总之，Nogoa中位于AA 634-668和AA 1026-1055的两个位置被我们的两个抗体识别，是中枢神经系统(CNS)损伤后修复的新的和潜在的潜在靶点。
In a previous study, we generated two monoclonal antibodies (mAbs) in mice, aNogoA-N and aNogo-66 mAb, which were raised against recombinant N-terminal fragments of rat NogoA and Nogo-66, respectively. When compared with the commercial rabbit anti-rat NogoA polyclonal antibody (pAb), which can specifically recognise NogoA, the two mAbs were also specific for the NogoA antigen in immunofluorescence histochemical (IHC) staining and Western blot (WB) analysis. Serial truncations of NogoA covering the N-terminal region of NogoA (aa 570–691) and Nogo-66 (aa 1026–1091) were expressed in E. coli. The epitopes recognised by aNogoA-N and aNogo-66 are located in the aa 634–668 and aa 1026–1055 regions of NogoA, respectively. Both mAbs remarkably enhanced the axon growth and branching of cultured hippocampal neurons in vitro. These results suggest that the antibodies that bind to aa 634–668 and aa 1026–1055 of NogoA may have stimulatory effects on axon growth and branching. Additionally, the two mAbs that we generated are specific for NogoA and significantly block NogoA function. In conclusion, two sites in NogoA located within aa 634–668 and aa 1026–1055 are recognised by our two antibodies and are novel and potentially promising targets for repair after central nervous system (CNS) injury.
登录
查看更多内容
抗Nogo-A抗体治疗促进成年灵长类单侧颈椎损伤后手灵巧度的恢复——行为数据的重新检查和扩展。
DOI：
10.1111/j.1460-9568.2009.06642.x
发表时间：
2009-03
期刊：
The European journal of neuroscience
影响因子：
--
作者：
Freund P;Schmidlin E;Wannier T;Bloch J;Mir A;Schwab ME;Rouiller EM
通讯作者：
Rouiller EM
Amino-Nogo-A 拮抗活性氧的产生并保护未成熟的初级皮质神经元免受氧化毒性
影响因子：
12.4
作者：
Mi, Y-J;Hou, B.;Jin, W-L
通讯作者：
Jin, W-L
影响因子：
14.5
作者：
Maier, Irin C.;Ichiyama, Ronaldo M.;Schwab, Martin E.
通讯作者：
Schwab, Martin E.
M9 是氨基-Nogo-A 的一个新区域，通过抑制小鼠 NADPH 氧化酶衍生的超氧化物产生来减轻脑缺血损伤
影响因子：
5.5
作者：
Guo, Fan;Jin, Wei-Lin;Xiong, Li-Ze
通讯作者：
Xiong, Li-Ze
影响因子：
16.2
作者：
Kim, JE;Liu, BP;Strittmatter, SM
通讯作者：
Strittmatter, SM