Effects of elevated carbon dioxide, elevated temperature, and rice growth stage on the community structure of rice root-associated bacteria.
Effects of elevated carbon dioxide, elevated temperature, and rice growth stage on the community structure of rice root-associated bacteria.
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DOI：
10.1264/jsme2.me14011
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发表时间：
2014
影响因子：
2.2
通讯作者：
Minamisawa K
中科院分区：
文献类型：
--
作者：
Okubo T;Tokida T;Ikeda S;Bao Z;Tago K;Hayatsu M;Nakamura H;Sakai H;Usui Y;Hayashi K;Hasegawa T;Minamisawa K
利用16S核糖体RNA基因克隆文库分析技术，研究了自由空气CO2浓度升高（FACE）和土壤水温升高（升温）对水稻根际细菌群落的影响。根取样穗分化和成熟阶段41和92天后移栽（DAT），分别。在92 DAT时，甲烷氧化菌Methylosinus和Methylocystis的相对丰度随温度升高而增加，而FACE则降低，表明水稻根系微生物甲烷氧化可能受到全球变暖的影响。Burkholderia kururiensis的相对丰度增加变暖在41 DAT和FACE或变暖在92 DAT。甲烷氧化菌的丰度增加水稻生长过程中，这可能是由增加从稻田的CH4排放，这表明CH4是影响水稻根中微生物群落结构的主导因素之一。在其他属的水稻生长过程中也观察到显着的变化在社区结构：慢生根瘤菌，梭菌，和一个未知的属接近Epsilonproteobacteria是丰富的在92 DAT，而无色杆菌是丰富的在41 DAT。这些结果表明，水稻根相关细菌的社区结构显着影响FACE，温度和水稻生长阶段。
The effects of free-air carbon dioxide enrichment (FACE) and elevated soil and water temperature (warming) on the rice root–associated bacterial community were evaluated by clone library analysis of the 16S ribosomal RNA gene. Roots were sampled at the panicle initiation and ripening stages 41 and 92 days after transplanting (DAT), respectively. The relative abundances of the methanotrophs Methylosinus and Methylocystis were increased by warming and decreased by FACE at 92 DAT, which indicated that microbial methane (CH4) oxidation in rice roots may have been influenced by global warming. The relative abundance of Burkholderia kururiensis was increased by warming at 41 DAT and by FACE or warming at 92 DAT. The abundances of methanotrophs increased during rice growth, which was likely induced by an enhancement in the emission of CH4 from the paddy fields, suggesting that CH4 is one of the predominant factors affecting the structure of the microbial community in rice roots. Marked variations in the community structure were also observed during rice growth in other genera: Bradyrhizobium, Clostridium, and an unknown genus close to Epsilonproteobacteria were abundant at 92 DAT, whereas Achromobacter was abundant at 41 DAT. These results demonstrated that the community structures of rice root-associated bacteria were markedly affected by FACE, temperature, and the rice growth stage.
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影响因子：
4.9
作者：
Ham, Jong Hyun;Melanson, Rebecca A.;Rush, Milton C.
通讯作者：
Rush, Milton C.
影响因子：
3
作者：
Hasegawa, Toshihiro;Sakai, Hidemitsu;Makino, Amane
通讯作者：
Makino, Amane
影响因子：
3.4
作者：
Graff, A;Stubner, S
通讯作者：
Stubner, S
影响因子：
4.4
作者：
Ashelford, Kevin E.;Chuzhanova, Nadia A.;Weightman, Andrew J.
通讯作者：
Weightman, Andrew J.
影响因子：
4.4
作者：
Schloss, Patrick D.;Westcott, Sarah L.;Weber, Carolyn F.
通讯作者：
Weber, Carolyn F.