Expression of zinc finger protein 105 in the testis and its role in male fertility.
Expression of zinc finger protein 105 in the testis and its role in male fertility.
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DOI：
10.1002/mrd.21171
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发表时间：
2010-06
影响因子：
2.5
通讯作者：
Lan, Zi-Jian
中科院分区：
文献类型：
--
作者：
Zhou, Huaxin;Liu, Lan-Hsin;Zhang, Heng;Lei, Zhenmin;Lan, Zi-Jian
使用计算机模拟的方法，我们确定了一个假定的锌指结构域的转录因子（锌指蛋白105，ZFP 105），是丰富的成年小鼠睾丸。RT-PCR分析表明，Zfp 105确实在成年小鼠睾丸中高表达，其表达在出生后发育过程中受到调节。为了进一步表征Zfp 105表达，我们产生了Zfp 105：β-半乳糖苷酶（LacZ）敲入报告基因小鼠系（Zfp 105 LacZ/+），其中表达Zfp 105：LacZ融合基因。成年Zfp 105 LacZ/+组织的整体包埋LacZ分析显示睾丸中有强LacZ染色，卵巢中有非常弱的染色，脾脏、肝脏、肾脏、心脏、肺、胸腺、肾上腺、子宫或输卵管中无染色。切片LacZ染色显示ZFP 105在粗线期精母细胞中高表达。ZFP 105的人类直系同源物ZNF 35也在正常人睾丸的雄性生殖细胞中表达。更重要的是，在成年Zfp 105 LacZ/LacZ小鼠中观察到雄性生育力降低和生精细胞脱落。综上所述，我们的研究结果表明，ZFP 105是一种男性生殖细胞因子，在男性生殖中发挥作用。
Using an in silico approach, we identified a putative zinc finger domain-containing transcription factor (zinc finger protein 105, ZFP105) that was enriched in the adult mouse testis. RT-PCR analyses showed that Zfp105 was indeed highly expressed in adult mouse testis and that its expression was regulated during postnatal development. To further characterize Zfp105 expression, we generated a Zfp105:β-galactosidase (LacZ) knock-in reporter mouse line (Zfp105LacZ/+) in which a Zfp105:LacZ fusion gene was expressed. Whole-mount LacZ analyses of adult Zfp105LacZ/+ tissues showed robust LacZ staining in the testis, very weak staining in the ovary and no staining in the spleen, liver, kidney, heart, lung, thymus, adrenal gland, uterus or oviduct. Sectional LacZ staining showed that ZFP105 was highly expressed in pachytene spermatocytes. ZNF35, the human ortholog of ZFP105, was also expressed in male germ cells of normal human testis. More importantly, reduced male fertility and sloughed spermatogenic cells were observed in adult Zfp105LacZ/LacZ mice. Taken together, our results suggest that ZFP105 is a male germ-cell factor and plays a role in male reproduction.
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影响因子：
64.8
作者：
KINZLER, KW;RUPPERT, JM;VOGELSTEIN, B
通讯作者：
VOGELSTEIN, B
影响因子：
4
作者：
Kimmins, S;Kotaja, N;Sassone-Corsi, P
通讯作者：
Sassone-Corsi, P
影响因子：
3.5
作者：
PENGUE, G;CANNADABARTOLI, P;LANIA, L
通讯作者：
LANIA, L
影响因子：
2.5
作者：
Przyborski, SA;Knowles, BB;Ackerman, SL
通讯作者：
Ackerman, SL
DOI：
10.1002/bdra.20131
发表时间：
2005-04-01
影响因子：
--
作者：
Okada, A;Kushima, K;Fujiwara, M
通讯作者：
Fujiwara, M