Expression of VEGF receptors on endothelial cells in mouse skeletal muscle.
Expression of VEGF receptors on endothelial cells in mouse skeletal muscle.
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DOI：
10.1371/journal.pone.0044791
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Popel AS
中科院分区：
文献类型：
--
作者：
Imoukhuede PI;Popel AS
VEGFR表面定位在将细胞外VEGF信号传导转化为血管生成结果中起着关键作用，并且这些参数的定量表征对于推进计算模型至关重要;然而，这些受体在血管上的水平目前尚不清楚。因此，我们的目的是定量测定血管内皮生长因子受体的定位从小鼠后肢骨骼肌内皮细胞。我们通过与体外细胞上的VEGFR水平进行比较来将这种VEGFR定量化。使用定量荧光，我们测量和比较从C57 BL/6和BALB/c腓肠肌和胫前后肢肌肉分离的内皮细胞上的VEGFR 1和VEGFR 2的水平。使用具有已知数目的藻红蛋白分子的珠校准荧光测量。数据显示VEGFR 1表面定位相对于VEGFR 2高2倍，其中VEGFR 1/内皮细胞为2，000 - 3，700，VEGFR 2/内皮细胞为1，300 - 2，000。我们确定，内皮细胞从高度糖酵解肌肉，胫骨前，含有30%以上的VEGFR 1表面受体的数量比腓肠肌; BALB/c小鼠显示17%以上的VEGFR 1的数量比C57 BL/6。当我们将这些结果与体外小鼠成纤维细胞进行比较时，我们观察到高水平的VEGFR 1（35，800/细胞）和非常低水平的VEGFR 2（700/细胞），而在体外人内皮细胞中，我们观察到VEGF的平衡被逆转，VEGFR 2水平较高（5，800/细胞），VEGFR 1水平较低（1，800/细胞）。我们的研究还揭示了受体表达的显著细胞间异质性，并且第一次离体定量这些差异提供了对抗血管生成或调节（VEGFR 1）和促血管生成（VEGFR 2）信号传导的平衡的了解。
VEGFR surface localization plays a critical role in converting extracellular VEGF signaling towards angiogenic outcomes, and the quantitative characterization of these parameters is critical for advancing computational models; however the levels of these receptors on blood vessels is currently unknown. Therefore our aim is to quantitatively determine the VEGFR localization on endothelial cells from mouse hindlimb skeletal muscles. We contextualize this VEGFR quantification through comparison to VEGFR-levels on cells in vitro. Using quantitative fluorescence we measure and compare the levels of VEGFR1 and VEGFR2 on endothelial cells isolated from C57BL/6 and BALB/c gastrocnemius and tibialis anterior hindlimb muscles. Fluorescence measurements are calibrated using beads with known numbers of phycoerythrin molecules. The data show a 2-fold higher VEGFR1 surface localization relative to VEGFR2 with 2,000–3,700 VEGFR1/endothelial cell and 1,300–2,000 VEGFR2/endothelial cell. We determine that endothelial cells from the highly glycolytic muscle, tibialis anterior, contain 30% higher number of VEGFR1 surface receptors than gastrocnemius; BALB/c mice display ∼17% higher number of VEGFR1 than C57BL/6. When we compare these results to mouse fibroblasts in vitro, we observe high levels of VEGFR1 (35,800/cell) and very low levels of VEGFR2 (700/cell), while in human endothelial cells in vitro, we observe that the balance of VEGFRs is inverted, with higher levels VEGFR2 (5,800/cell) and lower levels of VEGFR1 (1,800/cell). Our studies also reveal significant cell-to-cell heterogeneity in receptor expression, and the quantification of these dissimilarities ex vivo for the first time provides insight into the balance of anti-angiogenic or modulatory (VEGFR1) and pro-angiogenic (VEGFR2) signaling.
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影响因子：
56.9
作者：
Cukierman, E;Pankov, R;Yamada, KM
通讯作者：
Yamada, KM
DOI：
10.1115/1.3138276
发表时间：
1981-01-01
影响因子：
1.7
作者：
DEWEY, CF;BUSSOLARI, SR;DAVIES, PF
通讯作者：
DAVIES, PF
影响因子：
9.8
作者：
Bishop, E T;Bell, G T;Wheatley, D N
通讯作者：
Wheatley, D N
影响因子：
4.8
作者：
Bernatchez, Pascal N.;Acevedo, Lisette;Sessa, William C.
通讯作者：
Sessa, William C.
影响因子：
3.9
作者：
Chen, JH;Wang, XC;Sato, JD
通讯作者：
Sato, JD