Brain and Pituitary Transcriptome Analyses Reveal the Differential Regulation of Reproduction-Related LncRNAs and mRNAs in Cynoglossus semilaevis.
Brain and Pituitary Transcriptome Analyses Reveal the Differential Regulation of Reproduction-Related LncRNAs and mRNAs in Cynoglossus semilaevis.
复制标题
脑和垂体转录组分析揭示了半滑舌鱼繁殖相关 lncRNA 和 mRNA 的差异调节
DOI：
10.3389/fgene.2021.802953
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发表时间：
2021
影响因子：
3.7
通讯作者：
Shi B
中科院分区：
文献类型：
--
作者：
Dong Y;Lyu L;Wen H;Shi B
长非编码RNA(LncRNAs)参与了半滑舌板(Cynoglossus Semilaevis)的繁殖。然而，对它们在生殖中的作用的研究主要集中在卵巢上，而它们在大脑和脑下垂体中的表达模式和潜在作用尚不清楚。因此，为了探索在脑和垂体中与生殖密切相关的mRNAs和lncRNAs，我们分三个阶段采集舌底脑和垂体部组织进行RNA测序，分别在脑和垂体部鉴定出5,135和5,630个差异表达(DE)mRNAs和378和532个DE lncRNAs。RNA-seq扩增结果经RT-qPCR验证。此外，还对DEmRNAs和lncRNAs的功能进行了分析。有趣的是，它们参与了与新陈代谢、信号转导和内分泌信号相关的通路。基于反义、顺式和反式调控机制构建了LncRNA与靶基因的相互作用网络。此外，我们还构建了竞争的内源RNA(Cerna)网络。综上所述，本研究提供了脑和脑垂体中的mRNA和lncRNA的表达谱，以了解调控舌底繁殖的分子机制。
Long noncoding RNAs (lncRNAs) have been identified to be involved in half-smooth tongue sole (Cynoglossus semilaevis) reproduction. However, studies of their roles in reproduction have focused mainly on the ovary, and their expression patterns and potential roles in the brain and pituitary are unclear. Thus, to explore the mRNAs and lncRNAs that are closely associated with reproduction in the brain and pituitary, we collected tongue sole brain and pituitary tissues at three stages for RNA sequencing (RNA-seq), the 5,135 and 5,630 differentially expressed (DE) mRNAs and 378 and 532 DE lncRNAs were identified in the brain and pituitary, respectively. The RNA-seq results were verified by RT-qPCR. Moreover, enrichment analyses were performed to analyze the functions of DE mRNAs and lncRNAs. Interestingly, their involvement in pathways related to metabolism, signal transduction and endocrine signaling was revealed. LncRNA-target gene interaction networks were constructed based on antisense, cis and trans regulatory mechanisms. Moreover, we constructed competing endogenous RNA (ceRNA) networks. In summary, this study provides mRNA and lncRNA expression profiles in the brain and pituitary to understand the molecular mechanisms regulating tongue sole reproduction.
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影响因子：
4.1
作者：
Chang, John P.;Pemberton, Joshua G.
通讯作者：
Pemberton, Joshua G.
影响因子：
2.7
作者：
Gu, Weiwei;Yang, Yang;Xu, Shanliang
通讯作者：
Xu, Shanliang
DOI：
10.1007/978-3-319-42059-2_1
发表时间：
2016-01-01
期刊：
NON-CODING RNAS IN COLORECTAL CANCER
影响因子：
--
作者：
Hombach, Sonja;Kretz, Markus
通讯作者：
Kretz, Markus
影响因子：
12.3
作者：
Faghihi MA;Zhang M;Huang J;Modarresi F;Van der Brug MP;Nalls MA;Cookson MR;St-Laurent G 3rd;Wahlestedt C
通讯作者：
Wahlestedt C
影响因子：
5.3
作者：
Arun, Gayatri;Akhade, Vijay Suresh;Rao, Manchanahalli R. Satyanarayana
通讯作者：
Rao, Manchanahalli R. Satyanarayana