Growth characteristics of canine pathogenic viruses in MDCK cells cultured in RPMI 1640 medium without animal protein.
Growth characteristics of canine pathogenic viruses in MDCK cells cultured in RPMI 1640 medium without animal protein.
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DOI：
10.1016/j.vaccine.2005.07.114
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发表时间：
2006-03-10
期刊：
影响因子：
5.5
通讯作者：
Mochizuki M
中科院分区：
文献类型：
--
作者：
Mochizuki M
将MDCK细胞适应于无血清RPMI1640培养液，用于犬病毒的培养。在RPMI1640培养基中添加大豆蛋白胨、L-谷氨酰胺和抗生素，使蛋白质浓度低于5μg/ml(RPMISP培养基)。所获得的适应MDCK-SP细胞在RPMI/SP培养液(MDCK-SP细胞培养)第28代后表现出稳定的生长。犬瘟热病毒、犬细小病毒、犬腺病毒和犬副流感病毒是犬联合病毒疫苗的主要成分，它们在MDCK-SP细胞上的生长效率与在含有胎牛血清的常规Eagle‘s MEM中培养的MDCK细胞相同。因此，使用MDCK-SP细胞培养可以使目前的犬类疫苗产品更加安全，质量更高，成本更低。
Madin Darby canine kidney (MDCK) cells were adapted to serum-free RPMI 1640 medium and used for cultivation of canine viruses. RPMI 1640 medium was supplemented with a soybean peptone, l-glutamine and antibiotics, so that the protein concentration was less than 5 μg/ml (RPMI/SP medium). The resulting adapted MDCK-SP cells showed steady growth after the twenty-eighth passage in RPMI/SP medium (MDCK-SP cell culture). Canine distemper virus, canine parvovirus, canine adenoviruses and canine parainfluenza virus, which are the principal components of canine combined virus vaccines, grew in the MDCK-SP cell culture as efficiently as the parental MDCK cells cultured in the conventional Eagle's MEM containing fetal bovine serum. Consequently, the use of MDCK-SP cell culture can make current canine vaccine products much safer, of higher quality and at lower cost.
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影响因子：
5.4
作者：
Seki, F;Ono, N;Yanagi, Y
通讯作者：
Yanagi, Y
影响因子：
2.2
作者：
Merten, OW;Wu, R;Crainic, R
通讯作者：
Crainic, R
影响因子：
9.4
作者：
Mochizuki, M;Hashimoto, M;Carmichael, LE
通讯作者：
Carmichael, LE
影响因子：
2.2
作者：
Makoschey, B;Patel, JR;van Gelder, PTJA
通讯作者：
van Gelder, PTJA
DOI：
10.1007/bf01240073
发表时间：
1974-01-01
期刊：
ARCHIV FUR DIE GESAMTE VIRUSFORSCHUNG
影响因子：
--
作者：
JOHNSON, RH;SIEGL, G;GAUTSCHI, M
通讯作者：
GAUTSCHI, M