Effect of oligofructose on resistance to postoperative high-fat diet-induced damage of metabolism in diabetic rats after sleeve gastrectomy.
Effect of oligofructose on resistance to postoperative high-fat diet-induced damage of metabolism in diabetic rats after sleeve gastrectomy.
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低聚果糖对糖尿病大鼠袖状胃切除术后高脂饮食诱导代谢损伤抵抗力的影响
DOI：
10.4239/wjd.v12.i4.453
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发表时间：
2021-04-15
影响因子：
4.2
通讯作者：
Zhang GY
中科院分区：
文献类型：
--
作者：
Zhong MW;Li Y;Cheng YG;Liu QR;Hu SY;Zhang GY
背景：袖式胃切除术（SG）能显著缓解2型糖尿病。然而，糖尿病的长期缓解率通常会随着时间的推移而降低。低聚果糖已被证实可以调节宿主的代谢。本研究旨在探讨低聚果糖对高脂饮食（HFD）诱导的SG后代谢功能障碍的保护作用。目的探讨低聚果糖对糖尿病大鼠SG后糖尿病缓解的作用及其机制。方法对HFD、烟酰胺、低剂量链脲佐菌素诱导的糖尿病大鼠进行SG和SHAM手术。然后SHAM组和SG组大鼠连续给予HFD，袖胃切除术-低聚果糖组大鼠给予含有10%低聚果糖的特异性HFD。在指定时间点测定并比较体重、卡路里摄入量、口服葡萄糖耐量试验、胰岛素抵抗稳态模型评估、血脂、血清胰岛素、胰高血糖素样肽1 （GLP-1）、总胆汁酸、脂多糖（LPS）和结肠微生物群水平。所有统计分析均使用Statistic Package for Social Science version 19.0 （IBM, United States）进行，P < 0.05认为差异有统计学意义。结果术后2周，接受SG治疗的大鼠血糖和脂质代谢指标均有改善。与SG组相比,老鼠从SG-oligofructose组表现出更好的参数的葡萄糖和脂质代谢,降低体重(526.86±21.51 vs 469.25±21.84,P < 0.001),热量摄入(152.14±9.48 vs 129.63±8.99,P < 0.001),内稳态模型评估胰岛素抵抗(4.32±0.57 vs 3.46±0.52,P < 0.05),和LPS水平(0.19±0.01 vs 0.16±0.01,P < 0.05),和更高水平的胰岛素(1.17±0.17 vs 1.58±0.16,P < 0.001)、GLP-1（12.39±1.67 vs 14.94±1.86,P < 0.001）、双歧杆菌（0.0034±0.0014 vs 0.0343±0.0064,P < 0.001）、乳酸菌（0.0161±0.0037 vs 0.0357±0.0047,P < 0.001）、嗜muciniphila（0.0050±0.0024 vs 0.0507±0.0100,P < 0.001）的相对丰度。然而，两组之间的总胆汁酸水平没有观察到差异。结论低聚果糖可部分预防食油诱导的SG后糖脂代谢损伤，其机制可能与热量摄入、胰岛素、GLP-1、LPS及肠道菌群的改变有关。
BACKGROUND Sleeve gastrectomy (SG) can induce prominent remission of type 2 diabetes mellitus. However, the long-term remission rate of diabetes usually decreases over time. Oligofructose has been verified to modulate host metabolism. The aim of this study was to explore the protective effect of oligofructose on high-fat diet (HFD)-induced metabolic dysfunction after SG. AIM To study the effect and mechanism of oligofructose on diabetic remission in diabetic rats after SG. METHODS SG and SHAM operation were performed on diabetes rats induced with an HFD, nicotinamide, and low-dose streptozotocin. Then the rats in the SHAM and SG groups were continuously provided with the HFD, and the rats in sleeve gastrectomy-oligofructose group were provided with a specific HFD containing 10% oligofructose. Body weight, calorie intake, oral glucose tolerance test, homeostasis model assessment of insulin resistance, lipid profile, serum insulin, glucagon-like peptide 1 (GLP-1), total bile acids, lipopolysaccharide (LPS), and colonic microbiota levels were determined and compared at the designated time points. All statistical analyses were performed using Statistic Package for Social Science version 19.0 (IBM, United States), and the statistically significant difference was considered at P < 0.05. RESULTS At 2 wk after surgery, rats that underwent SG exhibited improved indexes of glucose and lipid metabolism. Compared with the SG group, the rats from SG-oligofructose group exhibited better parameters of glucose and lipid metabolism, lower body weight (526.86 ± 21.51 vs 469.25 ± 21.84, P < 0.001), calorie intake (152.14 ± 9.48 vs 129.63 ± 8.99, P < 0.001), homeostasis model assessment of insulin resistance (4.32 ± 0.57 vs 3.46 ± 0.52, P < 0.05), and LPS levels (0.19 ± 0.01 vs 0.16 ± 0.01, P < 0.05), and higher levels of insulin (1.17 ± 0.17 vs 1.58 ± 0.16, P < 0.001) and GLP-1 (12.39 ± 1.67 vs 14.94 ± 1.86, P < 0.001), and relative abundances of Bifidobacterium (0.0034 ± 0.0014 vs 0.0343 ± 0.0064, P < 0.001), Lactobacillus (0.0161 ± 0.0037 vs 0.0357 ± 0.0047, P < 0.001), and Akkermansia muciniphila (0.0050 ± 0.0024 vs 0.0507 ± 0.0100, P < 0.001) at the end of the study. However, no difference in total bile acids levels was observed between the two groups. CONCLUSION Oligofructose partially prevents HFD-induced glucose and lipid metabolism damage after SG, which may be due to the changes of calorie intake, insulin, GLP-1, LPS, and the gut microbiota in rats.
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