Electron capture dissociation of divalent metal-adducted sulfated N-glycans released from bovine thyroid stimulating hormone.
Electron capture dissociation of divalent metal-adducted sulfated N-glycans released from bovine thyroid stimulating hormone.
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DOI：
10.1007/s13361-013-0700-3
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发表时间：
2013-11
影响因子：
3.2
通讯作者：
Håkansson K
中科院分区：
文献类型：
--
作者：
Zhou W;Håkansson K
采用电喷雾电离（ESI）技术，将牛促甲状腺激素（bTSH）释放的硫酸n -聚糖与二价金属阳离子Ca2+、Mg2+和Co离子电离。对这些金属加合物进行了红外多光子离解（IRMPD）和电子俘获离解（ECD），并比较了相应的破碎模式。IRMPD产生广泛的糖苷和交叉环裂解，但大多数产物离子遭受磺酸盐损失。内部碎片也被观察到，这使光谱变得复杂。与IRMPD相比，ECD提供了互补的结构信息，所有观察到的产物离子都保留了磺酸基，允许磺酸定位。据我们所知，这项工作代表了ECD首次应用于从糖蛋白释放的金属加合硫酸化n -聚糖。由于IRMPD和ECD能够提供互补的结构信息，这两种策略的结合是一种有前途和有价值的聚糖结构表征工具。同时考察了不同金属离子的影响。由于钙加合物的高灵敏度和提供广泛结构信息的能力，它似乎是最有希望的物种。
Sulfated N-glycans released from bovine thyroid stimulating hormone (bTSH) were ionized with the divalent metal cations Ca2+, Mg2+, and Co by electrospray ionization (ESI). These metal-adducted species were subjected to infrared multiphoton dissociation (IRMPD) and electron capture dissociation (ECD) and the corresponding fragmentation patterns were compared. IRMPD generated extensive glycosidic and cross-ring cleavages, but most product ions suffered from sulfonate loss. Internal fragments were also observed, which complicated the spectra. ECD provided complementary structural information compared with IRMPD, and all observed product ions retained the sulfonate group, allowing sulfonate localization. To our knowledge, this work represents the first application of ECD towards metal-adducted sulfated N-glycans released from a glycoprotein. Due to the ability of IRMPD and ECD to provide complementary structural information, the combination of the two strategies is a promising and valuable tool for glycan structural characterization. The influence of different metal ions was also examined. Calcium adducts appeared to be the most promising species because of high sensitivity and ability to provide extensive structural information.
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DOI：
10.1016/s1044-0305(01)00268-9
发表时间：
2001-08-01
影响因子：
3.2
作者：
Harvey, DJ
通讯作者：
Harvey, DJ
DOI：
10.1002/oms.1210260527
发表时间：
1991-05-01
期刊：
ORGANIC MASS SPECTROMETRY
影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
7.4
作者：
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Zubarev, RA
DOI：
10.1016/j.jasms.2005.09.009
发表时间：
2006-01-01
影响因子：
3.2
作者：
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通讯作者：
Lebrilla, CB