Modification of Threonine-825 of SlBRI1 Enlarges Cell Size to Enhance Fruit Yield by Regulating the Cooperation of BR-GA Signaling in Tomato.
Modification of Threonine-825 of SlBRI1 Enlarges Cell Size to Enhance Fruit Yield by Regulating the Cooperation of BR-GA Signaling in Tomato.
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DOI：
10.3390/ijms22147673
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发表时间：
2021-07-18
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Wang S;Lv S;Zhao T;Jiang M;Liu D;Fu S;Hu M;Huang S;Pei Y;Wang X
油菜素内酯（Brassinosteroids, BRs）是一种促进生长的植物激素，可以通过外源微摩尔浓度施用或内源调控BR相关基因表达来有效发挥作用，因此，精确控制BR信号强度是挖掘BRs农业潜力的关键因素。brassinosteroids INSENSITIVE1 （brassinosteroids INSENSITIVE1, BRI1）是一种BR受体，是BR信号转导中的限速酶，SlBRI1各磷酸化位点的磷酸化对BR信号强度和植物特性有明显影响。最近研究表明，对番茄SlBRI1的磷酸化位点进行修饰可以不同程度地改善番茄的农艺性状；然而，SlBRI1磷酸化位点在番茄中的相关农艺潜力尚未得到充分开发。本研究对番茄SlBRI1磷酸化位点苏氨酸-825 （Thr-825）的生物学功能进行了研究。表型分析表明，与携带SlBRI1的番茄品系相比，表达非磷酸化Thr-825 （T825A）的SlBRI1转基因番茄品系由于细胞大小和产量的增加而表现出更大的植株尺寸，包括更高的株高、更粗的茎、更长的节间长度、更大的植株膨大、更重的果实和更大的果实。分子分析进一步表明，当SlBRI1在Thr-825位点去磷酸化时，SlBRI1的自磷酸化水平、BR信号和赤霉素（gibberellic acid， GA）信号水平升高。综上所述，结果表明，Thr-825的去磷酸化可以增强SlBRI1在BR信号传导中的功能，进而激活并协同GA信号传导刺激细胞伸长，从而导致植株更大，单株产量更高。这些结果也强调了SlBRI1磷酸化位点通过精确控制BR信号来选育高产番茄品种的农业潜力。
Brassinosteroids (BRs) are growth-promoting phytohormones that can efficiently function by exogenous application at micromolar concentrations or by endogenous fine-tuning of BR-related gene expression, thus, precisely controlling BR signal strength is a key factor in exploring the agricultural potential of BRs. BRASSINOSTEROID INSENSITIVE1 (BRI1), a BR receptor, is the rate-limiting enzyme in BR signal transduction, and the phosphorylation of each phosphorylation site of SlBRI1 has a distinct effect on BR signal strength and botanic characteristics. We recently demonstrated that modifying the phosphorylation sites of tomato SlBRI1 could improve the agronomic traits of tomato to different extents; however, the associated agronomic potential of SlBRI1 phosphorylation sites in tomato has not been fully exploited. In this research, the biological functions of the phosphorylation site threonine-825 (Thr-825) of SlBRI1 in tomato were investigated. Phenotypic analysis showed that, compared with a tomato line harboring SlBRI1, transgenic tomato lines expressing SlBRI1 with a nonphosphorylated Thr-825 (T825A) exhibited a larger plant size due to a larger cell size and higher yield, including a greater plant height, thicker stems, longer internodal lengths, greater plant expansion, a heavier fruit weight, and larger fruits. Molecular analyses further indicated that the autophosphorylation level of SlBRI1, BR signaling, and gibberellic acid (GA) signaling were elevated when SlBRI1 was dephosphorylated at Thr-825. Taken together, the results demonstrated that dephosphorylation of Thr-825 can enhance the functions of SlBRI1 in BR signaling, which subsequently activates and cooperates with GA signaling to stimulate cell elongation and then leads to larger plants and higher yields per plant. These results also highlight the agricultural potential of SlBRI1 phosphorylation sites for breeding high-yielding tomato varieties through precise control of BR signaling.
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