Active diffusion and advection in Drosophila oocytes result from the interplay of actin and microtubules.
Active diffusion and advection in Drosophila oocytes result from the interplay of actin and microtubules.
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DOI：
10.1038/s41467-017-01414-6
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发表时间：
2017-11-15
影响因子：
16.6
通讯作者：
Palacios IM
中科院分区：
文献类型：
--
作者：
Drechsler M;Giavazzi F;Cerbino R;Palacios IM
细胞内的运输通过被动和主动过程的微妙相互作用发生，包括扩散、定向运输和平流。尽管超分辨率显微镜取得了进展，但区分和量化这些过程是一项挑战，需要在拥挤、嘈杂的环境中跟踪快速移动的亚衍射物体。在这里，我们使用不同的对比机制的差分动态显微镜提供了一个彻底的特性在果蝇卵母细胞的动态。我们研究了囊泡的运动和难以捉摸的运动的细胞质F-肌动蛋白网格，一个已知的调节细胞质流量。我们发现，细胞质运动构成了定向运动和随机扩散的组合。虽然平流主要归因于微管，但我们发现主动扩散是由肌动蛋白细胞骨架驱动的，尽管它也被流动增强。我们还发现，一个重要的动态链接之间存在的囊泡和细胞质F-肌动蛋白运动，最近在小鼠卵母细胞中提出。细胞内转运是由定向转运、平流和扩散等过程的组合促进的。在这里，作者显微镜下观察了果蝇卵母细胞的动态，发现细胞骨架成分对平流和主动扩散的不同贡献。
Transport in cells occurs via a delicate interplay of passive and active processes, including diffusion, directed transport and advection. Despite progress in super-resolution microscopy, discriminating and quantifying these processes is a challenge, requiring tracking of rapidly moving, sub-diffraction objects in a crowded, noisy environment. Here we use differential dynamic microscopy with different contrast mechanisms to provide a thorough characterization of the dynamics in the Drosophila oocyte. We study the movement of vesicles and the elusive motion of a cytoplasmic F-actin mesh, a known regulator of cytoplasmic flows. We find that cytoplasmic motility constitutes a combination of directed motion and random diffusion. While advection is mainly attributed to microtubules, we find that active diffusion is driven by the actin cytoskeleton, although it is also enhanced by the flow. We also find that an important dynamic link exists between vesicles and cytoplasmic F-actin motion, as recently suggested in mouse oocytes. Intracellular transport is facilitated by a combination of processes including directed transport, advection and diffusion. Here the authors microscopically characterise the dynamics of the Drosophila oocyte and find distinct contributions of cytoskeletal components to advection and active diffusion.
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DOI：
10.1073/pnas.0703094104
发表时间：
2007-10-09
影响因子：
11.1
作者：
Brangwynne, Clifford P.;MacKintosh, F. C.;Weitz, David A.
通讯作者：
Weitz, David A.
影响因子：
56.9
作者：
Brendza, RP;Serbus, LR;Saxton, WM
通讯作者：
Saxton, WM
影响因子：
9.2
作者：
Glotzer, JB;Saffrich, R;Ephrussi, A
通讯作者：
Ephrussi, A
影响因子：
9.2
作者：
Forrest, KM;Gavis, ER
通讯作者：
Gavis, ER
影响因子：
11.8
作者：
Hurd, Thomas Ryan;Herrmann, Beate;Lehmann, Ruth
通讯作者：
Lehmann, Ruth