Generation of a focused poly(amino ether) library: polymer-mediated transgene delivery and gold-nanorod based theranostic systems.
Generation of a focused poly(amino ether) library: polymer-mediated transgene delivery and gold-nanorod based theranostic systems.
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作者：
Vu L;Ramos J;Potta T;Rege K
采用不同的低聚胺开环聚合两种缩水甘油醚，合成了21种阳离子聚氨基醚。平行筛选用于质粒DNA（pDNA）递送的聚合物，并将单个聚合物的转基因表达效力与25 kDa聚乙烯亚胺（PEI）的转基因表达效力进行比较，25 kDa聚乙烯亚胺（PEI）是聚合物介导的转基因递送的当前标准。从筛选中鉴定出在胰腺癌和前列腺癌细胞系中表现出比PEI更高的转基因表达的七种先导聚合物。在MIA PaCa-2胰腺癌细胞系中，所有七种先导聚合物在聚合物：pDNA重量比为5：1时显示最高的转基因表达。在所研究的条件中，转基因表达功效与最小的聚合物细胞毒性相关，但与聚合物复合物的大小无关。此外，这项研究表明，在单体中的胺中心之间的亚甲基间距，胺含量，和聚合物的分子量都是重要的因素，并应考虑当设计用于转基因传递的聚合物。铅有效的聚合物被用于涂覆金纳米棒，导致治疗诊断纳米组装体，其具有组合的转基因递送和光学成像能力，从而导致潜在的治疗诊断系统。
A focused library of twenty-one cationic poly(amino ethers) was synthesized following ring-opening polymerization of two diglycidyl ethers by different oligoamines. The polymers were screened in parallel for plasmid DNA (pDNA) delivery, and transgene expression efficacies of individual polymers were compared to those of 25 kDa polyethylenimine (PEI), a current standard for polymer-mediated transgene delivery. Seven lead polymers that demonstrated higher transgene expression than PEI in pancreatic and prostate cancer cells lines were identified from the screen. All seven lead polymers showed highest transgene expression at a polymer:pDNA weight ratio of 5:1 in the MIA PaCa-2 pancreatic cancer cell line. Among the conditions studied, transgene expression efficacy correlated with minimal polymer cytotoxicity but not polyplex sizes. In addition, this study indicated that methylene spacing between amine centers in the monomers, amine content, and molecular weight of the polymers are all significant factors and should be considered when designing polymers for transgene delivery. A lead effective polymer was employed for coating gold nanorods, leading to theranostic nanoassemblies that possess combined transgene delivery and optical imaging capabilities, leading to potential theranostic systems.
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影响因子：
14
作者：
Guo, Shutao;Huang, Yuanyu;Wei, Tuo;Zhang, Wendi;Wang, Weiwei;Lin, Daoshu;Zhang, Xu;Kumar, Anil;Du, Quan;Xing, Jinfeng;Deng, Liandong;Liang, Zicai;Wang, Paul C.;Dong, Anjie;Liang, Xing-Jie
通讯作者：
Liang, Xing-Jie
影响因子：
10.8
作者：
Durr, Nicholas J.;Larson, Timothy;Ben-Yakar, Adela
通讯作者：
Ben-Yakar, Adela
影响因子：
--
作者：
Emilitri, E;Ranucci, E;Ferruti, P
通讯作者：
Ferruti, P
影响因子：
3
作者：
Ghaemy, M;Barghamadi, M;Behmadi, H
通讯作者：
Behmadi, H
影响因子：
5.1
作者：
Jean, M.;Alameh, M.;Merzouki, A.
通讯作者：
Merzouki, A.