Expression of MUC1 mucin in full-term pregnancy human placenta.
Expression of MUC1 mucin in full-term pregnancy human placenta.
复制标题
MUC1粘蛋白在足月妊娠人胎盘中的表达。
DOI：
10.2478/v10039-008-0017-9
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发表时间：
2008
影响因子：
2.7
通讯作者：
Średzińska K
中科院分区：
文献类型：
--
作者：
A. Paszkiewicz;Porowska H;Średzińska K
目的
MUC 1粘蛋白是人子宫内膜糖萼的一种成分，在胚胎着床期“接受窗”的形成中起重要作用。考虑到MUC 1在人胎盘中的表达在妊娠期间发生变化，并且MUC 1的结构和功能在该器官中并不完全已知，我们已经进行了这种粘蛋白的分离和聚糖表位的检测，因为它们对其性质至关重要。
材料和方法
将人胎盘样品均质化，并使用离子或非离子洗涤剂在不同条件下提取MUC 1。经Sepharose 4 B柱纯化后，用Western和凝集素印迹法进行鉴定。
结果
MUC 1糖蛋白的最佳提取是用非离子去污剂Triton X-100实现的。与抗MUC 1抗体的反应显示出分子量在116和205 kDa之间的一些糖型，最明显的糖型接近205 kDa。与凝集素的反应使得能够检测碳水化合物抗原，例如具有通过α 2，3键连接的唾液酸的T和Tn，并且在较小程度上通过α 2，6键连接。
结论
MUC 1粘蛋白在足月分娩后以几种糖型存在于人胎盘的母体侧。它们含有短聚糖结构，类似于一些肿瘤碳水化合物抗原。
PURPOSE
MUC1 mucin is a component of glycocalyx in human endometrium and may play an important role in generation of "receptive window" at embryo implantation. Considering that MUC1 expression in human placenta is changed during pregnancy, and that MUC1 structure and function are not completely known in this organ, we have undertaken isolation of this mucin and detection of glycan epitopes, since they are crucial for its properties.
MATERIAL AND METHODS
Samples of human placenta were homogenized and MUC1 was extracted in different conditions with the use of ionic or non-ionic detergents. Identification of this glycoprotein was performed by Western and lectin blotting, after its purification on Sepharose 4B column.
RESULTS
The best extraction of MUC1 glycoprotein was achieved with a non-ionic detergent, Triton X-100. Reactions with anti-MUC1 antibody showed a few glycoforms with molecular weights between 116 and 205 kDa, with the most visible glycoform approximating 205 kDa. Reactions with lectins enabled detection of carbohydrate antigens, such as T and Tn with sialic acid linked by alpha2, 3 and to a lesser extent by alpha2, 6 bond.
CONCLUSION
MUC1 mucin is present in several glycoforms on the maternal side of human placenta after term delivery. They contain short glycan structures, similar to some tumor carbohydrate antigens.
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影响因子：
3.4
作者：
DeSouza, MM;Surveyor, GA;Carson, DD
通讯作者：
Carson, DD
影响因子：
4.6
作者：
C. Damsky;C. Librach;K. Lim;M. L. Fitzgerald;M. Mcmaster;M. Janatpour;Yan Zhou;S. Logan;S. Fisher
通讯作者：
C. Damsky;C. Librach;K. Lim;M. L. Fitzgerald;M. Mcmaster;M. Janatpour;Yan Zhou;S. Logan;S. Fisher
DOI：
10.1016/j.bbamcr.2007.04.006
发表时间：
2007-06-01
影响因子：
5.1
作者：
Thirkill, Twanda L.;Cao, Tim;Douglas, Gordon C.
通讯作者：
Douglas, Gordon C.
黄体酮和植入囊胚调节兔子宫上皮中 Muc1 的表达。
DOI：
10.1210/endo.139.1.5750
发表时间：
1998
期刊：
Endocrinology.
影响因子：
--
作者：
Hoffman,LH;Olson,GE;Carson,DD;Chilton,BS
通讯作者：
Chilton,BS