A postinfluenza model of Staphylococcus aureus pneumonia.
A postinfluenza model of Staphylococcus aureus pneumonia.
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DOI：
10.1086/650204
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发表时间：
2010-02-15
期刊：
影响因子：
--
通讯作者：
Lowy FD
中科院分区：
文献类型：
--
作者：
Lee MH;Arrecubieta C;Martin FJ;Prince A;Borczuk AC;Lowy FD
流感后金黄色葡萄球菌肺炎越来越被认为是危及生命的感染的主要形式。开发了流感后金黄色葡萄球菌肺炎的小鼠模型。小鼠经鼻内单独感染细菌或细菌加病毒。通过小鼠存活率、肺组织病理学、肺部细菌密度和细胞对感染的反应来评估感染。与仅感染金黄色葡萄球菌的小鼠相比，同时感染流感病毒和金黄色葡萄球菌的小鼠表现出更高的死亡率、更大的肺实质损伤以及转移组织部位更高的细菌密度。 4小时后，与感染细菌的小鼠相比，感染病毒和细菌的小鼠的肺样本中发现更多的多形核白细胞和更少的CD11c+细胞。 α-溶血素和蛋白A在感染后4小时表达最大，Panton-Valentine杀白细胞素在感染后72小时表达最大，单独感染细菌的小鼠α-溶血素表达水平较高。从感染病毒加细菌的小鼠收集的组织中，干扰素 γ 的表达高于感染细菌的小鼠的组织。该模型的结果证明了先前的流感病毒感染引起的多种影响，对金黄色葡萄球菌肺炎相关的发病率和死亡率具有深远的影响。
Postinfluenza Staphylococcus aureus pneumonias are increasingly recognized as a major form of life-threatening infections. A mouse model of postinfluenza S. aureus pneumonia was developed. Mice were intranasally infected with bacteria alone or bacteria plus virus. Infection was assessed by mouse survival, lung histopathology, bacterial density in the lungs, and cellular response to infection. Mice infected with both influenza virus and S. aureus showed higher mortality, greater lung parenchymal damage, and greater bacterial density at metastatic tissue sites than mice infected with only S. aureus. At 4 h, more polymorphonuclear leukocytes and fewer CD11c+ cells were found in lung samples from mice infected with virus and bacteria than in those from mice infected with bacteria. α-Hemolysin and protein A were maximally expressed 4 h after infection, and Panton-Valentine leukocidin was maximally expressed 72 h after infection, with higher levels of α-hemolysin expression in mice infected with bacteria alone. Interferon γ expression was higher in tissue collected from mice infected with virus plus bacteria than in those from bacteria-infected mice. The results from this model demonstrate diverse effects caused by antecedent influenza virus infection, which have a profound influence on the morbidity and mortality associated with S. aureus pneumonia.
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DOI：
10.1016/s1473-3099(06)70466-2
发表时间：
2006-05
期刊：
The Lancet. Infectious diseases
影响因子：
--
作者：
Brundage JF
通讯作者：
Brundage JF
DOI：
10.1086/591708
发表时间：
2008-10-01
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Morens DM;Taubenberger JK;Fauci AS
通讯作者：
Fauci AS
DOI：
10.1128/aem.16.4.588-594.1968
发表时间：
1968-01-01
期刊：
APPLIED MICROBIOLOGY
影响因子：
--
作者：
GAUSH, CR;SMITH, TF
通讯作者：
SMITH, TF
影响因子：
6.4
作者：
Morens, David M.;Fauci, Anthony S.
通讯作者：
Fauci, Anthony S.
影响因子：
6.4
作者：
McCullers, JA;Bartmess, KC
通讯作者：
Bartmess, KC