Signal-mediated nuclear transport in proliferating and growth-arrested BALB/c 3T3 cells.
Signal-mediated nuclear transport in proliferating and growth-arrested BALB/c 3T3 cells.
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DOI：
10.1083/jcb.115.4.933
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发表时间：
1991-11
期刊：
影响因子：
--
通讯作者：
Akin D
中科院分区：
文献类型：
--
作者：
Feldherr CM;Akin D
通过分析核纤溶酶包被的胶体金注入细胞质后的核摄取，研究了增殖和生长停滞(融合或血清饥饿)的BALB/c 3T3细胞的跨核转运。与增殖细胞相比，在10、14、17和21天的融合培养中，大颗粒(直径110-270A，包括蛋白外壳)的核摄取分别减少5.5、33和78倍，但对小颗粒(总直径50-80A)的相对摄取不随细胞年龄的增加而减少。这一发现表明，在汇合的种群中，基本上所有的毛孔都保持着功能，但大多数毛孔失去了运输大颗粒的能力。通过注入中等大小的金颗粒，在14-17和21天的培养中，降级毛孔中运输通道的功能直径估计分别约为130和110埃。在增殖细胞中，转运通道的功能直径约为230A。在10%的水平上，增殖细胞和融合细胞的平均孔径(膜到膜的距离)(分别为728和712A)有显著差异，但在5%的水平上差异不显著。在孔密度(单位长度的膜孔)方面没有发现差异。去血清细胞(在1%血清中7-8天或在0.5%血清中4天)对大而不小的金颗粒的核摄取也显著减少。因此，渗透性效应不仅仅是高细胞密度的函数，而且似乎与生长有关。讨论了这些发现可能的功能意义。
Mediated transport across the nuclear envelope was investigated in proliferating and growth-arrested (confluent or serum starved) BALB/c 3T3 cells by analyzing the nuclear uptake of nucleoplasmin-coated colloidal gold after injection into the cytoplasm. Compared with proliferating cells the nuclear uptake of large gold particles (110-270 A in diameter, including the protein coat) decreased 5.5-, 33-, and 78- fold, respectively, in 10-, 14-17-, and 21-d-old confluent cultures; however, the relative uptake of small particles (total diameter 50-80 A) did not decrease with increasing age of the cells. This finding suggests that essentially all pores remain functional in confluent populations, but that most pores lose their capacity to transport large particles. By injecting intermediate-sized gold particles, the functional diameters of the transport channels in the downgraded pores were estimated to be approximately to 130 and 110 A, in 14-17- and 21-d- old cultures, respectively. In proliferating cells, the transport channels have a functional diameter of approximately 230 A. The mean diameters of the pores (membrane-to-membrane distance) in proliferating and confluent cells (728 and 712 A, respectively) were significantly different at the 10%, but not the 5%, level. No differences in pore density (pore per unit length of membrane) were detected. Serum- deprived cells (7-8 d in 1% serum or 4 d in 0.5% serum) also showed a significant decrease in the nuclear uptake of large, but not small, gold particles. Thus, the permeability effects are not simply a function of high cell density but appear to be growth related. The possible functional significance of these findings is discussed.
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影响因子：
11.4
作者：
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通讯作者：
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影响因子：
7.8
作者：
Adam, S A;Marr, R S;Gerace, L
通讯作者：
Gerace, L