Differential expression of circulating microRNAs in blood and haematoma samples from patients with intracerebral haemorrhage.
Differential expression of circulating microRNAs in blood and haematoma samples from patients with intracerebral haemorrhage.
复制标题
脑出血患者血液和血肿样本中循环 microRNA 的差异表达。
DOI：
10.1177/0300060516630852
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发表时间：
2016-06
期刊：
影响因子：
--
通讯作者：
He Z
中科院分区：
文献类型：
--
作者：
Wang J;Zhu Y;Jin F;Tang L;He Z;He Z
测定脑出血（ICH）患者外周血样本中microRNA（miRNA）的差异表达，并测定ICH患者外周血和血肿样本中hsa-miR-21- 5 p的水平。该病例对照研究招募了接受开颅手术治疗的壳核ICH患者和年龄和性别匹配的健康对照受试者。使用miRNA聚合酶链反应（PCR）阵列测定患者组和对照组的血清miRNA表达谱。选择ICH相关的miRNA hsa-miR-21- 5 p，并使用实时PCR评估其在ICH患者的外周血和血肿标本中与外周血样本对照相比的差异表达。7名患者和5名对照受试者被纳入miRNA表达谱分析; 31名患者和22名对照受试者提供了用于hsa-miR-21- 5 p表达的实时PCR样品。共有59个miRNAs在ICH患者中显著下调。与对照受试者相比，患者组外周血和血肿样本的相对hsa-miR-21- 5 p水平分别为0.43和0.31。在ICH患者的外周血和血肿样本中，Hsa-miR-21- 5 p水平均显著降低。
To measure the differential expression of microRNAs (miRNAs) in peripheral blood samples from patients with intracerebral haemorrhage (ICH) and to measure the levels of hsa-miR-21-5p in peripheral blood and haematoma samples from patients with ICH. This case–control study enrolled individuals with ICH in the putamen treated by craniotomy and age- and sex-matched healthy control subjects. Serum miRNA expression profiles were determined in the patient and control groups using miRNA polymerase chain reaction (PCR) arrays. The ICH-related miRNA hsa-miR-21-5p was selected and its differential expression was assessed in peripheral blood and haematoma specimens from patients with ICH compared with peripheral blood samples controls using real-time PCR. Seven patients and five control subjects were included in the miRNA expression profile analysis; and 31 patients and 22 control subjects provided samples for the real-time PCR of hsa-miR-21-5p expression. A total of 59 miRNAs were significantly downregulated in patients with ICH. Relative hsa-miR-21-5p levels of 0.43 and 0.31 for peripheral blood and haematoma samples, respectively, were obtained in the patient group compared with the control subjects. Hsa-miR-21-5p levels were significantly reduced in both peripheral blood and haematoma samples in patients with ICH.
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影响因子：
56.9
作者：
Lagos-Quintana, M;Rauhut, R;Tuschl, T
通讯作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.0800121105
发表时间：
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影响因子：
11.1
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82.9
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