Prevalence and Characterization of Carbapenem-Hydrolyzing Class D β-Lactamase-Producing Acinetobacter Isolates From Ghana.
Prevalence and Characterization of Carbapenem-Hydrolyzing Class D β-Lactamase-Producing Acinetobacter Isolates From Ghana.
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DOI：
10.3389/fmicb.2020.587398
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发表时间：
2020
影响因子：
5.2
通讯作者：
Saito R
中科院分区：
文献类型：
--
作者：
Ayibieke A;Kobayashi A;Suzuki M;Sato W;Mahazu S;Prah I;Mizoguchi M;Moriya K;Hayashi T;Suzuki T;Iwanaga S;Ablordey A;Saito R
不动杆菌属细菌的多药耐药性，特别是碳青霉烯类耐药性是全球医疗保健关注的问题。然而，关于西非（包括加纳）不动杆菌属分离株的表型和基因型特征的现有数据很少。我们的目的是调查来自加纳的不动杆菌属分离株的抗生素耐药性和基因型特征，并使用全基因组测序（WGS）来表征碳青霉烯酶生产者。分析了2016年至2017年期间在加纳三家医院收集的36株不动杆菌分离株。通过商业抗生素平板测定MIC。使用巴斯德方案测定鲍曼不动杆菌MLST。使用短读和长读测序策略对OXA-碳青霉烯酶生产者进行WGS。耐药率最高的是甲氧苄啶/磺胺甲恶唑（n = 22; 61%）。分别有6株（16.7%）和8株（22.2%）对头孢他啶和粘菌素耐药。2株（5.6%）对3种碳青霉烯类抗生素耐药，1株（2.8%）对3种碳青霉烯类抗生素中度敏感。24 A中发现了15个ST。鲍曼不动杆菌分离株，包括6个新ST（ST 1467和ST 1472）。ST 78是主要的（n = 6），其次是ST 1469（n = 3）。4株产碳青霉烯酶A.还鉴定了鲍曼不动杆菌分离株。同基因ST 103分离株Ab-B 004 d-c和Ab-D10 a-a在相同质粒pAb-B 004 d-c_3和pAb-D10 a-a_3上的Tn 2007内携带blaOXA-23。ST 1472分离株Ab-C102和ST 107分离株Ab-C63分别携带blaOXA-58和blaOXA-420（一种罕见的blaOXA-58变异体），存在新的遗传背景。结果表明，A.不同和独特基因型的鲍曼不动杆菌分离株，包括OXA-碳青霉烯酶生产者，正在加纳流行，这突出表明需要更广泛地监测抗菌素耐药性。
Multidrug resistance, especially carbapenem resistance in Acinetobacter bacteria is a global healthcare concern. However, available data on the phenotypic and genotypic characteristics of Acinetobacter isolates from West Africa, including Ghana is scanty. Our aim was to investigate the antibiotic resistance profile and genotypic characteristics of Acinetobacter isolates from Ghana and to characterize carbapenemase producers using whole-genome sequencing (WGS). A total of 36 Acinetobacter isolates collected at three hospitals in Ghana between 2016 and 2017 were analyzed. MICs were determined by commercial antibiotic plates. Acinetobacter baumannii MLST was determined using the Pasteur scheme. WGS of OXA-carbapenemase producers was performed using short- and long-read sequencing strategies. The resistance rate was highest for trimethoprim/sulfamethoxazole (n = 22; 61%). Six (16.7%) and eight (22.2%) isolates were resistant to ceftazidime and colistin, respectively. Two (5.6%) isolates were resistant and one (2.8%) isolate had intermediate sensitivity to three carbapenems. Fifteen STs were identified in 24 A. baumannii isolates including six new STs (ST1467 ∼ ST1472). ST78 was the predominant (n = 6) followed by ST1469 (n = 3). Four carbapenemase-producing A. baumannii isolates also were identified. Isogenic ST103 isolates Ab-B004d-c and Ab-D10a-a harbored blaOXA–23 within Tn2007 on identical plasmids, pAb-B004d-c_3, and pAb-D10a-a_3. ST1472 isolate Ab-C102 and ST107 isolate Ab-C63 carried blaOXA–58 and blaOXA–420, a rare blaOXA–58 variant, respectively, within novel genetic contexts. Our results show that A. baumannii isolates of diverse and unique genotypes, including OXA-carbapenemase producers, are circulating in Ghana highlighting the need for a wider surveillance of antimicrobial resistance.
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影响因子：
14.9
作者：
Galata V;Fehlmann T;Backes C;Keller A
通讯作者：
Keller A
影响因子：
4.9
作者：
Chen, Te-Li;Wu, Roy Chen-Chih;Cho, Wen-Long
通讯作者：
Cho, Wen-Long
影响因子：
9.4
作者：
Higgins, Paul G.;Lehmann, Marlene;Seifert, Harald
通讯作者：
Seifert, Harald
影响因子：
14.2
作者：
Ghazawi, A.;Sonnevend, A.;Pal, T.
通讯作者：
Pal, T.
影响因子：
5.2
作者：
Cameranesi MM;Morán-Barrio J;Limansky AS;Repizo GD;Viale AM
通讯作者：
Viale AM