Regulation of amino acid transporters in the mammary gland from late pregnancy to peak lactation in the sow.
Regulation of amino acid transporters in the mammary gland from late pregnancy to peak lactation in the sow.
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DOI：
10.1186/s40104-018-0250-4
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发表时间：
2018
影响因子：
7
通讯作者：
Guan W
中科院分区：
文献类型：
--
作者：
Chen F;Zhang S;Deng Z;Zhou Q;Cheng L;Kim SW;Chen J;Guan W
乳蛋白质是影响母猪乳质量和仔猪健康的重要物质基础。母猪血浆氨基酸是乳腺合成乳蛋白的重要前体物质。为了研究AA在母猪乳腺中转运的调控及其可能的机制，我们测定了3个不同生理阶段母猪乳蛋白编码基因的表达、AA转运蛋白的表达和血浆AA浓度（哺乳期第17天、第1天和第17天），并以猪乳腺上皮细胞（PMEC）为体外模型，进一步研究了AA跨膜转运的适应性调节机制。将PMEC培养在含有4种氨基酸浓度（0 × AA复合物、1 × AA复合物、5 × AA复合物和25 × AA复合物）的DMEM：F12中。本研究中评估的AA复合物类别包括中性AA（L-Ala + L-Ser + L-Cys）、酸性AA（L-Asp、L-Glu）和中性+碱性AA（L-Ala + L-Ser + L-Cys + L-Lys）。我们的结果表明，编码乳蛋白基因的mRNA表达，αs1-酪蛋白、αs2-酪蛋白、β-酪蛋白和κ-酪蛋白含量随生理期的推进而显著增加（P < 0.05），以及包括苏氨酸、丝氨酸、谷氨酸、丙氨酸、缬氨酸、半胱氨酸、甲硫氨酸、异亮氨酸和酪氨酸在泌乳D1时高于泌乳D17和D17（P < 0.05）。此外，泌乳母猪乳腺中兴奋性AA转运体3（EAAT 3）、丙氨酸/丝氨酸/半胱氨酸/苏氨酸转运体1（ASCT 1）和钠偶联中性AA转运体2（SNAT 2）的蛋白和基因表达量均高于妊娠后期（P <0. 05）。与DMEM/F12细胞培养液相比，添加5 ×和25 × AA复合物的细胞培养液中SLC 38 A2、SLC 1A 1、SLC 6A 14的mRNA表达量显著增加（P < 0.05）。与DMEM/F12相比，SLC 38 A、SLC 1A 4和SLC 6A 14的mRNA表达在EBSS细胞培养基中也增加。而在EBSS中加入AA复合物后，SLC 38 A的mRNA表达下降（P < 0.05）。从妊娠后期到泌乳高峰，随着生理阶段的推进，母猪乳腺中AA的转运受到正向调节，PMEC中的AA转运体受到AA浓度的适应性调节。
Milk protein is crucial for milk quality in sows and health of newborn piglets. Plasma amino acids (AA) in sows are important precursors for milk protein synthesis in the mammary gland. In order to study the regulation of AA transported in sow mammary glands and possible underlying mechanisms, we measured the expression of genes coding for milk proteins, AA transporter expressions, and plasma AA concentrations in sows at three different physiological stages (D-17, D1 and D17 of lactation), and then further investigated the regulation of AA transport across the cell membrane by adaptive mechanisms using pig mammary epithelial cells (PMEC) as an in vitro model. PMEC were cultured in DMEM:F12 with 4 amino acid concentrations (0 × AA complex, 1 × AA complex, 5 × AA complex, and 25 × AA complex). Classes of AA complexes evaluated in this study included neutral AAs (L-Ala + L-Ser + L-Cys), acidic AAs (L-Asp, L-Glu) and neutral + basic AAs (L-Ala + L-Ser + L-Cys + L-Lys). Our results indicated that mRNA expression of genes coding for milk protein (αs1-casein, αs2-casein, β-casein and κ-casein) increased significantly with the advance of physiological stage (P < 0.05), and plasma concentrations of most AAs including threonine, serine, glutamate, alanine, valine, cysteine, methionine, isoleucine and tyrosine were greater at D1 of lactation compared with D-17 and D17 of lactation (P < 0.05). Additionally, protein and gene expressions of AA transporters including excitatory AA transporter 3 (EAAT3), alanine/serine/cysteine/threonine transporter (ASCT1) and sodium-coupled neutral AA transporter 1 (SNAT2) were greater in lactating sow mammary glands compared with sow mammary glands in late pregnancy (P < 0.05). The mRNA expressions of SLC38A2, SLC1A1, SLC6A14 increased significantly in the cell mediums supplemented with 5 × and 25 × of AA complexes compared with those cells cultured in DMEM/F12 cell medium (P < 0.05). The mRNA expressions of SLC38A, SLC1A4, and SLC6A14 also increased in EBSS cell medium compared to DMEM/F12. However, only mRNA expression of SLC38A decreased when AA complex was added into EBSS (P < 0.05). AA transportation was positively regulated in sow mammary glands with the advance of physiological stage from late pregnancy to peak of lactation and AA transporters in PMECs were adaptively regulated by changed AA concentrations.
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影响因子：
3.3
作者：
Ji, F;Hurley, WL;Kim, SW
通讯作者：
Kim, SW
DOI：
10.1016/0005-2736(72)90097-1
发表时间：
1972-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
GAZZOLA, GC;GUIDOTTI, GG;FRANCHI, R
通讯作者：
FRANCHI, R
影响因子：
2.5
作者：
Peaker, M
通讯作者：
Peaker, M
影响因子：
4.2
作者：
Guan, XF;Bequette, BJ;Trottier, NL
通讯作者：
Trottier, NL
影响因子：
2.9
作者：
Finucane, Kiera A.;McFadden, Thomas B.;Zhao, Feng-Qi
通讯作者：
Zhao, Feng-Qi