SKPs derive from hair follicle precursors and exhibit properties of adult dermal stem cells.
SKPs derive from hair follicle precursors and exhibit properties of adult dermal stem cells.
复制标题
DOI：
10.1016/j.stem.2009.10.019
复制
发表时间：
2009-12-04
期刊：
影响因子：
23.9
通讯作者：
Miller FD
中科院分区：
文献类型：
--
作者：
Biernaskie J;Paris M;Morozova O;Fagan BM;Marra M;Pevny L;Miller FD
尽管哺乳动物皮肤具有显着的再生能力，但尚未鉴定出成体真皮干细胞。在这里，我们研究了皮肤源性前体（SKP）是否可以发挥这样的作用。我们证明 SKP 源自 Sox2+ 毛囊真皮细胞，并且这两个细胞群在转录组和功能特性方面相似。克隆 SKP 和内源性 Sox2+ 细胞均可诱导毛发形态发生、分化为真皮细胞类型，并在移植后归入毛囊生态位。此外，毛囊衍生的 SKP 能够自我更新，保持其多能性，并连续重建毛囊。最后，移植实验表明，毛囊相关真皮细胞移出其生态位，为真皮维护和伤口愈合提供细胞。因此，SKP 源自 Sox2+ 毛囊相关真皮前体，并显示出真皮干细胞的预测功能特性，有助于真皮维护、伤口愈合和毛囊形态发生。
Despite the remarkable regenerative capacity of mammalian skin, an adult dermal stem cell has not yet been identified. Here, we investigated whether skin-derived precursors (SKPs) might fulfill such a role. We show that SKPs derive from Sox2+ hair follicle dermal cells, and that these two cell populations are similar with regard to their transcriptome and functional properties. Both clonal SKPs and endogenous Sox2+ cells induce hair morphogenesis, differentiate into dermal cell types, and home to a hair follicle niche upon transplantation. Moreover, hair follicle-derived SKPs self-renew, maintain their multipotency, and serially reconstitute hair follicles. Finally, grafting experiments show that follicle-associated dermal cells move out of their niche to contribute cells for dermal maintenance and wound-healing. Thus, SKPs derive from Sox2+ follicle-associated dermal precursors and display functional properties predicted of a dermal stem cell, contributing to dermal maintenance, wound-healing, and hair follicle morphogenesis.
登录
查看更多内容
影响因子：
21.3
作者：
Fernandes, KJL;McKenzie, IA;Miller, FD
通讯作者：
Miller, FD
影响因子：
2.6
作者：
Hadjantonakis, AK;Gertsenstein, M;Nagy, A
通讯作者：
Nagy, A
影响因子：
6.5
作者：
Zheng, Y;Du, XB;Stenn, KS
通讯作者：
Stenn, KS
影响因子：
56.9
作者：
Nishimura, EK;Granter, SR;Fisher, DE
通讯作者：
Fisher, DE
影响因子：
5.2
作者：
Hunt, David P. J.;Morris, Paul N.;Chandran, Siddharthan
通讯作者：
Chandran, Siddharthan