The form and function of channelrhodopsin.
The form and function of channelrhodopsin.
复制标题
DOI：
10.1126/science.aan5544
复制
发表时间：
2017-09-15
期刊：
影响因子：
--
通讯作者：
Hegemann P
中科院分区：
文献类型：
--
作者：
Deisseroth K;Hegemann P
通道视紫红质是一种光控离子通道，通过调节鞭毛功能，使单细胞活动藻类能够寻求适合光合作用和生存的环境光条件。这些植物的行为反应最初是在150多年前研究的。最近，光门控离子通道的主要功能原理已经通过创建具有加速或减慢数量级动力学的通道视紫红质，通过发现和设计具有改变光谱特性的通道视紫红质，通过解决高分辨率通道视紫红质晶体结构，以及通过结构模型指导的通道视紫红质重新设计改变离子选择性来阐明。这些发现不仅揭示了控制光门控离子通道运作的基本原理，而且还通过光遗传学创造了新的蛋白质，用于照亮大脑功能的基础。（上）左：通道视紫红质的内部工作。右图：结构导向重新设计产生的新型光子脉冲转导模式。（下）发现抑郁症相关症状的因果基础。如图所示，哺乳动物多巴胺神经元驱动的奖励状态（左）的脑区域特异性活动动态被前额皮质（右）抑制，使用第二和第三光子脉冲转导模式。
Channelrhodopsins are light-gated ion channels that, via regulation of flagellar function, enable single-celled motile algae to seek ambient light conditions suitable for photosynthesis and survival. These plant behavioral responses were initially investigated more than 150 years ago. Recently, major principles of function for light-gated ion channels have been elucidated by creating channelrhodopsins with kinetics that are accelerated or slowed over orders of magnitude, by discovering and designing channelrhodopsins with altered spectral properties, by solving the high-resolution channelrhodopsin crystal structure, and by structural model–guided redesign of channelrhodopsins for altered ion selectivity. Each of these discoveries not only revealed basic principles governing the operation of light-gated ion channels, but also enabled the creation of new proteins for illuminating, via optogenetics, the fundamentals of brain function. (Top) Left: Inner workings of channelrhodopsin. Right: New photon-spike transduction modes arising from structure-guided redesign. (Bottom) Discovering the causal underpinnings of depression-related symptomatology. Brain region–specific activity dynamics of the mammalian dopamine neuron–driven reward state (left) are suppressed by the prefrontal cortex (right) as shown, using the second and third photon-spike transduction modes.
登录
查看更多内容
影响因子：
62.1
作者：
Ernst, Oliver P.;Lodowski, David T.;Elstner, Marcus;Hegemann, Peter;Brown, Leonid S.;Kandori, Hideki
通讯作者：
Kandori, Hideki
DOI：
10.1098/rstb.1999.0541
发表时间：
1999-12-29
期刊：
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
Crick, F
通讯作者：
Crick, F
影响因子：
25
作者：
Banghart, M;Borges, K;Kramer, RH
通讯作者：
Kramer, RH
影响因子：
25
作者：
Boyden, ES;Zhang, F;Deisseroth, K
通讯作者：
Deisseroth, K
影响因子：
56.9
作者：
FARBER, IC;GRINVALD, A
通讯作者：
GRINVALD, A