Single-cell profiling of Anopheles gambiae spermatogenesis defines the onset of meiotic silencing and premeiotic overexpression of the X chromosome.
Single-cell profiling of Anopheles gambiae spermatogenesis defines the onset of meiotic silencing and premeiotic overexpression of the X chromosome.
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DOI：
10.1038/s42003-023-05224-z
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发表时间：
2023-08-15
影响因子：
5.9
通讯作者：
Galizi, Roberto
中科院分区：
文献类型：
--
作者：
Page, Nicole;Taxiarchi, Chrysanthi;Tonge, Daniel;Kuburic, Jasmina;Chesters, Emily;Kriezis, Antonios;Kyrou, Kyros;Game, Laurence;Nolan, Tony;Galizi, Roberto
了解冈比亚按蚊生殖系的发育和遗传调控对于设计针对这种疟疾蚊子媒介的有效遗传控制策略至关重要。这些包括靶向生殖系以诱导不育或使用调控序列驱动转基因表达以用于诸如基因驱动的应用。然而，只有很少的生殖系特异性调控元件的特点与大多数显示泄漏的表达。这已被证明大大降低了目前的遗传控制策略的效率，这些策略依赖于具有更严格限制的空间和/或时间表达的调控元件。性染色体的减数分裂沉默限制了转基因表达的灵活性，以开发有效的性连锁遗传控制策略。在这里，我们建立在我们以前的研究基础上，将配子发生解剖成四个不同的细胞群，使用单细胞RNA测序来定义八个不同的细胞簇和相关的种系细胞类型，使用可用的标记基因。我们揭示了X-连锁基因在一个独特的集群前减数分裂细胞的过度表达和文件的减数分裂沉默的X染色体在精子发生的后期阶段的细胞亚群的发病。这项研究提供了一个全面的数据集，通过精子发生表征不同细胞类型的表达，并扩大了疟疾蚊子遗传控制的工具包。scRNA-seq数据集揭示了蚊子和疟疾载体冈比亚按蚊雄性生殖系中单个细胞类型的转录组学特征。
Understanding development and genetic regulation in the Anopheles gambiae germline is essential to engineer effective genetic control strategies targeting this malaria mosquito vector. These include targeting the germline to induce sterility or using regulatory sequences to drive transgene expression for applications such as gene drive. However, only very few germline-specific regulatory elements have been characterised with the majority showing leaky expression. This has been shown to considerably reduce the efficiency of current genetic control strategies, which rely on regulatory elements with more tightly restricted spatial and/or temporal expression. Meiotic silencing of the sex chromosomes limits the flexibility of transgene expression to develop effective sex-linked genetic control strategies. Here, we build on our previous study, dissecting gametogenesis into four distinct cell populations, using single-cell RNA sequencing to define eight distinct cell clusters and associated germline cell–types using available marker genes. We reveal overexpression of X-linked genes in a distinct cluster of pre-meiotic cells and document the onset of meiotic silencing of the X chromosome in a subcluster of cells in the latter stages of spermatogenesis. This study provides a comprehensive dataset, characterising the expression of distinct cell types through spermatogenesis and widening the toolkit for genetic control of malaria mosquitoes. A scRNA-seq dataset reveals the transcriptomic profile of individual cell types in the male germline of the mosquito and malaria vector, Anopheles gambiae.
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影响因子：
64.8
作者：
Cao, Junyue;Spielmann, Malte;Shendure, Jay
通讯作者：
Shendure, Jay
DOI：
10.1111/j.1469-185x.1931.tb01030.x
发表时间：
1931-10-01
影响因子：
--
作者：
Fisher, RA
通讯作者：
Fisher, RA
影响因子：
4.6
作者：
Cheng, Jun;Tiyaboonchai, Amita;Hunt, Alan J.
通讯作者：
Hunt, Alan J.
影响因子：
16.6
作者：
Galizi R;Doyle LA;Menichelli M;Bernardini F;Deredec A;Burt A;Stoddard BL;Windbichler N;Crisanti A
通讯作者：
Crisanti A
影响因子：
6.6
作者：
Adashev，Vladimir E.;Bazylev，Sergei S.;Kotov，Alexei A.
通讯作者：
Kotov，Alexei A.