Grafting aptamers onto gold nanostars increases in vitro efficacy in a wide range of cancer cell types.
Grafting aptamers onto gold nanostars increases in vitro efficacy in a wide range of cancer cell types.
复制标题
将适体嫁接到金纳米级体上会在广泛的癌细胞类型中提高体外功效。
DOI：
10.1021/mp4005657
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发表时间：
2014-02-03
影响因子：
4.9
通讯作者：
Odom TW
中科院分区：
文献类型：
--
作者：
Dam DH;Culver KS;Odom TW
我们报告了一种纳米结构的设计，它可以通过靶向无处不在的蛋白质核仁素作为细胞类型独立的代理。金纳米星（AuNS）负载高密度的核仁特异性DNA适体AS 1411（Apt-AuNS）在一组12个癌症细胞系中产生抗癌作用，其中包含4个代表性的亚类。我们发现纳米结构可以被癌细胞内化并运输到核周区域。Apt-AuNS导致ca.与没有纳米结构的细胞相比是200%。用Apt-AuNS处理的癌细胞中的半胱天冬酶3/7活性（凋亡）和细胞死亡增加了1.5倍，并且增加了约1.5倍。与用超过10倍浓度的游离AS 1411处理的细胞相比，分别为17%。此外，从AuNS中光触发的适体释放进一步增强了纳米构建体在癌细胞系组中的体外功效，与仅用Apt-AuNS处理相比，半胱天冬酶活性增加2倍，细胞活力降低40%。相比之下，对一组正常细胞系进行有或没有光触发释放的纳米结构的处理没有不良影响。
We report the design of a nanoconstruct that can function as a cell-type independent agent by targeting the ubiquitous protein nucleolin. Gold nanostars (AuNS) loaded with high densities of nucleolin-specific DNA aptamer AS1411 (Apt-AuNS) produced anticancer effects in a panel of 12 cancer lines containing four representative subcategories. We found that the nanoconstructs could be internalized by cancer cells and trafficked to perinuclear regions. Apt-AuNS resulted in downregulation of antiapoptotic Bcl-2 mRNA expression by ca. 200% compared to cells without the nanoconstructs. The caspase 3/7 activity (apoptosis) and cell death in cancer cells treated with Apt-AuNS increased by 1.5 times and by ca. 17%, respectively, compared to cells treated with free AS1411 at over 10 times the concentration. Moreover, light-triggered release of aptamer from the AuNS further enhanced the in vitro efficacy of the nanoconstructs in the cancer line panel with a 2-fold increase in caspase activity and a 40% decrease in cell viability compared to treatment with Apt-AuNS only. In contrast, treatments of the nanoconstructs with or without light-triggered release on a panel of normal cell lines had no adverse effects.
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影响因子：
3.7
作者：
Hovanessian AG;Soundaramourty C;El Khoury D;Nondier I;Svab J;Krust B
通讯作者：
Krust B
影响因子：
64.8
作者：
Sawyers, C
通讯作者：
Sawyers, C
影响因子：
5.7
作者：
Girvan, Allicia C.;Teng, Yun;Bates, Paula J.
通讯作者：
Bates, Paula J.
影响因子：
11.2
作者：
Nahta, R;Hung, MC;Esteva, FJ
通讯作者：
Esteva, FJ
DOI：
10.1073/pnas.91.14.6569
发表时间：
1994-07-05
影响因子：
11.1
作者：
LAM, M;DUBYAK, G;DISTELHORST, CW
通讯作者：
DISTELHORST, CW