Syngenic grafting of a whole juvenile male gonadal tissue into the adult testes confers successful spermatogenesis in mice
Syngenic grafting of a whole juvenile male gonadal tissue into the adult testes confers successful spermatogenesis in mice
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将整个幼年雄性性腺组织同基因移植到成年睾丸中，使小鼠成功发生精子
DOI：
10.1016/j.apjr.2016.06.007
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发表时间：
2016
影响因子：
--
通讯作者：
Masahiro Sato
中科院分区：
文献类型：
--
作者：
N. Tada;Fumio Kanai;Eri Nakamura;Hongmei Lu;Masahiro Sato
目的观察从新生小鼠身上分离的雄性性腺组织（睾丸、附睾和脂肪）移植到成年小鼠睾丸下能否获得功能精子。方法从增强绿色荧光蛋白（EGFP）转基因（Tg）小鼠（C57BL/6-Tg(ACTB-EGFP)1Osb/J）中分离出1日龄雄性性腺组织，通过白膜切口植入非Tg受体小鼠睾丸深部。移植2个月后，从受体小鼠体内取出荧光移植组织。结果组织学分析显示附睾结构发育良好，在所有移植组织中，30-60%的精小管发生精子。有趣的是，在获得的7例移植中，有1例可以成功地从与附睾尾相对应的部分获得活动精子。这些获得的精子具有转基因，当使用冷冻解冻的精子进行卵胞浆内单精子注射时，可以支持胚胎发育。结论该技术可应用于具有产后致死性表型的Tg系和出生后立即死亡的克隆动物的抢救。
ObjectiveTo examine whether functional spermatozoa can be obtained when a whole male gonadal tissue (testes, epididymides, and fat) isolated from neonatal mice is grafted underneath adult mouse testes.MethodsNeonatal (1-day-old) male gonadal tissue, isolated from enhanced green fluorescent protein (EGFP)-transgenic (Tg) mice (C57BL/6-Tg(ACTB-EGFP)1Osb/J), was inserted deep in the testis of a non-Tg recipient mouse through a tunica albuginea incision. Two months after transplantation, the fluorescent grafted tissues were retrieved from recipient mice.ResultsHistological analysis demonstrated that epididymal architecture was well developed and that spermatogenesis in the testis occurred in 30–60% of each seminiferous tubule of all the grafted tissues examined. Interestingly, motile spermatozoa could be successfully retrieved from the portion corresponding to the cauda epididymis in 1 of the 7 transplants obtained. These obtained spermatozoa had transgenes and could support embryonic development when intracytoplasmic sperm injection was performed using frozen-thawed spermatozoa.ConclusionThis present technique will be useful for study in various biological fields including the rescue of Tg lines with lethal postnatal phenotypes and cloned animals that die immediately after birth.
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影响因子：
3.6
作者：
KIMURA, Y;YANAGIMACHI, R
通讯作者：
YANAGIMACHI, R
影响因子：
3.6
作者：
CHATOT, CL;LEWIS, JL;ZIOMEK, CA
通讯作者：
ZIOMEK, CA
DOI：
10.1530/jrf.0.0860679
发表时间：
1989-07-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
CHATOT, CL;ZIOMEK, CA;TORRES, I
通讯作者：
TORRES, I
DOI：
10.1073/pnas.91.24.11303
发表时间：
1994-11-22
影响因子：
11.1
作者：
BRINSTER, RL;AVARBOCK, MR
通讯作者：
AVARBOCK, MR