Technological advances in super-resolution microscopy to study cellular processes.

Technological advances in super-resolution microscopy to study cellular processes.
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DOI:
10.1016/j.molcel.2021.12.022
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发表时间:
2022-01-20
期刊:
影响因子:
16
通讯作者:
Lakadamyali M
Lakadamyali M
中科院分区:
生物学1区
文献类型:
--
作者:
Bond C;Santiago-Ruiz AN;Tang Q;Lakadamyali M

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自2000年首次展示以来,远场超分辨率光学显微镜经历了巨大的技术发展。与此同时,这些发展为以前所未有的细节水平可视化细胞的内部生活打开了一扇新的窗口。在这里,我们回顾了超分辨率显微镜最常用的实现背后的技术细节,并强调了该领域最近的一些有前途的进展。Bond等人提供了一个技术上的重点审查常见的超分辨率光学显微镜的方法,以及最近,有前途的技术发展在这一领域。该审查特别强调了新兴的先进成像方法,有望实现高时空分辨率的细胞分子结构的可视化。
Since its initial demonstration in 2000, far-field super-resolution light microscopy has undergone tremendous technological developments. In parallel, these developments have opened a new window into visualizing the inner life of cells at unprecedented levels of detail. Here, we review the technical details behind the most commonly used implementations of super-resolution microscopy and highlight some of the recent, promising advances in this field. Bond et al provide a technologically focused review on common super-resolution light microscopy approaches as well as recent, promising technical developments in this field. The review particularly highlights emerging advanced imaging approaches that hold promise for visualizing the molecular architecture of cells with high spatiotemporal resolution.
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