Evaluation of the Calu-3 cell line as a model of in vitro respiratory syncytial virus infection.
Evaluation of the Calu-3 cell line as a model of in vitro respiratory syncytial virus infection.
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DOI：
10.1016/j.jviromet.2011.03.027
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发表时间：
2011-06
影响因子：
3.1
通讯作者：
Haynes LM
中科院分区：
文献类型：
--
作者：
Harcourt JL;Caidi H;Anderson LJ;Haynes LM
呼吸道合胞病毒（RSV）的复制主要限于上呼吸道上皮，因此，原代分化的正常人支气管上皮细胞（NHBE）被认为是体外分析肺组织对呼吸道病毒感染和病毒-宿主相互作用反应的良好系统。然而，NHBE细胞是昂贵的，难以培养，并随来源患者而变化。另一种方法是使用具有支气管上皮细胞特征的连续细胞系，如Calu-3，一种来源于人肺腺癌的上皮细胞系，作为呼吸道病毒感染的体外模型。结果表明，Calu-3在作为液体覆盖的培养物的可渗透支持物上生长时完全可渗透。极化的Calu-3对RSV感染敏感，并主要从顶端表面释放感染性病毒，这与NHBE细胞中的研究一致。这些数据表明，极化Calu-3可以作为一个有用的体外模型来研究宿主对RSV感染的反应。
Respiratory syncytial virus (RSV) replication is primarily limited to the upper respiratory tract epithelium and primary, differentiated normal human bronchial epithelial cells (NHBE) have, therefore, been considered a good system for in vitro analysis of lung tissue response to respiratory virus infection and virus–host interactions. However, NHBE cells are expensive, difficult to culture, and vary with the source patient. An alternate approach is to use a continuous cell line that has features of bronchial epithelial cells such as Calu-3, an epithelial cell line derived from human lung adenocarcinoma, as an in vitro model of respiratory virus infection. The results show that Calu-3 fully polarize when grown on permeable supports as liquid-covered cultures. Polarized Calu-3 are susceptible to RSV infection and release infectious virus primarily from the apical surface, consistent with studies in NHBE cells. The data demonstrate that polarized Calu-3 may serve as a useful in vitro model to study host responses to RSV infection.
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影响因子：
5.4
作者：
ANDERSON, LJ;BINGHAM, P;HIERHOLZER, JC
通讯作者：
HIERHOLZER, JC
DOI：
10.1073/pnas.2434327100
发表时间：
2003-12-09
影响因子：
11.1
作者：
Brock, SC;Goldenring, JR;Crowe, JE
通讯作者：
Crowe, JE
影响因子：
6.4
作者：
HALL, CB;WALSH, EE;SCHNABEL, KC
通讯作者：
SCHNABEL, KC
影响因子：
5.9
作者：
Couch, Robert B.;Englund, Janet A.;Whimbey, Estella
通讯作者：
Whimbey, Estella
影响因子：
105.7
作者：
FERRIS, JAJ;AHERNE, WA;GARDNER, PS
通讯作者：
GARDNER, PS