Staphylococcus aureus Floating Biofilm Formation and Phenotype in Synovial Fluid Depends on Albumin, Fibrinogen, and Hyaluronic Acid.
Staphylococcus aureus Floating Biofilm Formation and Phenotype in Synovial Fluid Depends on Albumin, Fibrinogen, and Hyaluronic Acid.
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DOI：
10.3389/fmicb.2021.655873
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发表时间：
2021
影响因子：
5.2
通讯作者：
Hickok NJ
中科院分区：
文献类型：
--
作者：
Knott S;Curry D;Zhao N;Metgud P;Dastgheyb SS;Purtill C;Harwood M;Chen AF;Schaer TP;Otto M;Hickok NJ
生物膜通常在细菌培养基中进行研究，从而可以研究细菌生长等重要特性。然而，在此类培养基中获得的结果无法考虑体内细菌-蛋白质相互作用引起的细菌定位/聚类以及随之而来的表型、毒力因子产生和最终抗生素耐受性的改变。我们和其他人报道了耐甲氧西林或甲氧西林敏感的金黄色葡萄球菌（分别为 MRSA 或 MSSA）和其他病原体在滑液中组装蛋白质基质。这种蛋白质细菌聚集体被多糖基质包裹，这是生物膜的特征。在这项研究中，我们鉴定了对于这种聚集很重要的蛋白质，并确定了这些蛋白质可以重现细菌聚集的浓度范围。然后，我们测试了这种蛋白质组合引起显着聚集、抗菌耐受性、形态保存和酚溶性调节蛋白 (PSM) 毒力因子表达的能力。在此过程中，我们创建了一种粘性液体来模拟滑液中的细菌行为。我们认为，我们的发现以及这种液体的扩展使用可以帮助更好地模拟新抗菌疗法的细菌行为，并作为研究生理液体的宿主蛋白-细菌相互作用特征的起点。
Biofilms are typically studied in bacterial media that allow the study of important properties such as bacterial growth. However, the results obtained in such media cannot take into account the bacterial localization/clustering caused by bacteria–protein interactions in vivo and the accompanying alterations in phenotype, virulence factor production, and ultimately antibiotic tolerance. We and others have reported that methicillin-resistant or methicillin-susceptible Staphylococcus aureus (MRSA or MSSA, respectively) and other pathogens assemble a proteinaceous matrix in synovial fluid. This proteinaceous bacterial aggregate is coated by a polysaccharide matrix as is characteristic of biofilms. In this study, we identify proteins important for this aggregation and determine the concentration ranges of these proteins that can reproduce bacterial aggregation. We then test this protein combination for its ability to cause marked aggregation, antibacterial tolerance, preservation of morphology, and expression of the phenol-soluble modulin (PSM) virulence factors. In the process, we create a viscous fluid that models bacterial behavior in synovial fluid. We suggest that our findings and, by extension, use of this fluid can help to better model bacterial behavior of new antimicrobial therapies, as well as serve as a starting point to study host protein–bacteria interactions characteristic of physiological fluids.
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影响因子：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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