Cadherin-bound beta-catenin feeds into the Wnt pathway upon adherens junctions dissociation: evidence for an intersection between beta-catenin pools.
Cadherin-bound beta-catenin feeds into the Wnt pathway upon adherens junctions dissociation: evidence for an intersection between beta-catenin pools.
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DOI：
10.1371/journal.pone.0004580
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Quaranta V
中科院分区：
文献类型：
--
作者：
Kam Y;Quaranta V
β-连环蛋白是两个细胞系统的重要组成部分：基于钙粘附素的黏附连接(AJ)和Wnt信号通路。这些β-连环蛋白池之间的功能或物理联系在以前的研究中已经被提出，但到目前为止还没有确凿的证据。为了进一步研究这一交叉，我们用溶血磷脂酸(LPA)处理A431细胞克隆，它迫使AJ快速和同步解离。结合免疫染色、光敏绿色荧光蛋白标记的β-连环蛋白的时间推移显微镜和图像分析表明，β-连环蛋白结合的钙粘素池在AJ解离时与E-钙粘连蛋白一起内化在核周内胞再循环室，并可在Wnt途径激活时移位到细胞核内。这些结果表明，eRc可能是β-catenin进入细胞核的驻留部位，AJ的解离可能影响eRc中β-catenin的水平，从而有效地影响WnT途径下游可获得的β-catenin底物水平。这种交集提供了一种整合细胞间黏附和Wnt信号的机制，在依赖于β连环蛋白依赖的基因表达的发育和癌症过程中可能是至关重要的。
β-catenin is an essential component of two cellular systems: cadherin-based adherens junctions (AJ) and the Wnt signaling pathway. A functional or physical connection between these β-catenin pools has been suggested in previous studies, but not conclusively demonstrated to date. To further examine this intersection, we treated A431 cell colonies with lysophosphatidic acid (LPA), which forces rapid and synchronized dissociation of AJ. A combination of immunostaining, time-lapse microscopy using photoactivatable-GFP-tagged β-catenin, and image analyses indicate that the cadherin-bound pool of β-catenin, internalized together with E-cadherin, accumulates at the perinuclear endocytic recycling compartment (ERC) upon AJ dissociation, and can be translocated into the cell nucleus upon Wnt pathway activation. These results suggest that the ERC may be a site of residence for β-catenin destined to enter the nucleus, and that dissociation of AJ may influence β-catenin levels in the ERC, effectively affecting β-catenin substrate levels available downstream for the Wnt pathway. This intersection provides a mechanism for integrating cell-cell adhesion with Wnt signaling and could be critical in developmental and cancer processes that rely on β-catenin-dependent gene expression.
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DOI：
10.1007/bf02008338
发表时间：
1972-01-01
期刊：
EXPERIENTIA
影响因子：
--
作者：
HUOT, J;NOSAL, G;RADOUCOT.S
通讯作者：
RADOUCOT.S
影响因子：
10.5
作者：
Brembeck, FH;Schwarz-Romond, T;Birchmeier, W
通讯作者：
Birchmeier, W
影响因子：
64.8
作者：
Bhanot, P;Brink, M;Nusse, R
通讯作者：
Nusse, R
影响因子：
4
作者：
Bryant, David M.;Kerr, Markus C.;Stow, Jennifer L.
通讯作者：
Stow, Jennifer L.
DOI：
10.1083/jcb.200402153
发表时间：
2004-10-25
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gottardi CJ;Gumbiner BM
通讯作者：
Gumbiner BM